Protein Info for Pf1N1B4_922 in Pseudomonas fluorescens FW300-N1B4

Annotation: Sarcosine oxidase beta subunit (EC 1.5.3.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 69% identical to DAUA_PSEAE: FAD-dependent catabolic D-arginine dehydrogenase DauA (dauA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 90% identity to pba:PSEBR_a4581)

MetaCyc: 69% identical to catabolic D-arginine dehydrogenase (Pseudomonas aeruginosa)
RXN-7563 [EC: 1.4.99.6]

Predicted SEED Role

"Sarcosine oxidase beta subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

glycine betaine degradation I (6/8 steps found)
D-arginine degradation (2/3 steps found)
creatinine degradation I (1/4 steps found)
creatinine degradation II (1/5 steps found)

KEGG Metabolic Maps

Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.4.99.6 or 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161YZP6 at UniProt or InterPro

Protein Sequence (375 amino acids)

>Pf1N1B4_922 Sarcosine oxidase beta subunit (EC 1.5.3.1) (Pseudomonas fluorescens FW300-N1B4)
MSQADFIIIGGGIAGASTGFWLSQHGRVIVLERESHPAYHSTGRSAALFTAAYGTPQVRA
LTQASRDFFDAPPPGFCEHPLLTPRGEMTVDFTGDPAELSNQYLSAKATVPQMQWLSADE
ACARLPILRREKVHGAIYDPTASDIDTDALHQGYLRGIRRNKGEVHTDSEVLSLSRDAEG
QWQVQTATQTFSAPIIINAAGAWADKIGELAGAQPLGVQPKRRAAFIFAGPEGLDIHDWP
MLVSLDESFYMKPDAGMFLGSPANADPVEPHDVQPEELDIAMGIYQIEEATTLSIRRPTR
TWAGLRSFVRDGDLLSGFDQQVPGLFWVAAQGGYGIQTSPAMGQASAALVRGEALPEQLS
RFGLDAEMLSPARLS