Protein Info for Pf1N1B4_903 in Pseudomonas fluorescens FW300-N1B4

Annotation: Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 340 PF05971: Methyltransf_10" amino acids 27 to 324 (298 residues), 433.1 bits, see alignment E=6.1e-134 PF05175: MTS" amino acids 131 to 220 (90 residues), 25.5 bits, see alignment E=9e-10

Best Hits

Swiss-Prot: 92% identical to RLMF_PSEPF: Ribosomal RNA large subunit methyltransferase F (rlmF) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K06970, ribosomal RNA large subunit methyltransferase F [EC: 2.1.1.181] (inferred from 92% identity to pfo:Pfl01_0985)

Predicted SEED Role

"Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51)" (EC 2.1.1.51)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.51

Use Curated BLAST to search for 2.1.1.181 or 2.1.1.51

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MWQ5 at UniProt or InterPro

Protein Sequence (340 amino acids)

>Pf1N1B4_903 Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51) (Pseudomonas fluorescens FW300-N1B4)
MNAPRTPRPARKKPDSATPAKAVEPREKASLHPRNRHQGRYDFPALIKTTPELAKFVIIN
PYGKESIDFASPDAVRVFNRALLKSFYGIAHWDIPADYLCPPVPGRADYVHFLADLLASV
NDGEIPRGAPVKVLDIGMGANCVYPLIGYSDYRWHFLGSEIDPTAVAAAKAIVQSNGLNK
AIQLRQQSNPKHILLGLLEPGERFDLTMCNPPFHASMDEATKGSERKWRALGRADPKRKL
PVLNFGGQSAELWCEGGEARFVTQLIAESAHFQHKVLWFSTLVSKASNLPAIQTALKKAG
VLESQVVEMSQGQKQSRFVAWTFQTKSEQQVWRERWARKS