Protein Info for Pf1N1B4_899 in Pseudomonas fluorescens FW300-N1B4

Annotation: DNA polymerase III chi subunit (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 64% identical to HOLC_PSEAE: DNA polymerase III subunit chi (holC) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02339, DNA polymerase III subunit chi [EC: 2.7.7.7] (inferred from 94% identity to pfo:Pfl01_0988)

Predicted SEED Role

"DNA polymerase III chi subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162ART1 at UniProt or InterPro

Protein Sequence (142 amino acids)

>Pf1N1B4_899 DNA polymerase III chi subunit (EC 2.7.7.7) (Pseudomonas fluorescens FW300-N1B4)
MTKVDFYILPSADPSARLDFACKLTEKAWRMGHRIYLHCSDAAQRDDLDARLWAFKGESF
VPHGPAESEPEGLIVLGLGDDCGQHQDLLVNLDLKVPVFAKQFARVAEVVVEDPTIRSAA
RESFRFYREQGYPLQDHRLQRL