Protein Info for Pf1N1B4_895 in Pseudomonas fluorescens FW300-N1B4

Annotation: Translation elongation factor LepA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00009: GTP_EFTU
  • amino acids 6 to 184 (179 residues), 179.7 bits, E=1.2e-56
• TIGR01393: elongation factor 4
  • amino acids 6 to 597 (592 residues), 962.8 bits, E=6.2e-294
• TIGR00231: small GTP-binding protein domain
  • amino acids 9 to 176 (168 residues), 66.7 bits, E=2.1e-22
• PF00071: Ras
  • amino acids 67 to 183 (117 residues), 25.3 bits, E=2.6e-09
• PF03144: GTP_EFTU_D2
  • amino acids 208 to 278 (71 residues), 34.9 bits, E=4.3e-12
• PF00679: EFG_C
  • amino acids 403 to 487 (85 residues), 67.4 bits, E=2.3e-22
• PF06421: LepA_C
  • amino acids 490 to 596 (107 residues), 169.5 bits, E=5.1e-54

Best Hits

Swiss-Prot: 98% identical to LEPA_PSEFS: Elongation factor 4 (lepA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 98% identity to pfs:PFLU1058)

MetaCyc: 73% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A161Z4Z5 at UniProt or InterPro

Protein Sequence (599 amino acids)

>Pf1N1B4_895 Translation elongation factor LepA (Pseudomonas fluorescens FW300-N1B4)
VSDLSHIRNFSIIAHIDHGKSTLADRFIQMCGGLAEREMEAQVLDSMDLERERGITIKAH
SVTLYYTARDGIKYQLNFIDTPGHVDFTYEVSRSLAACEGALLVVDAGQGVEAQSVANCY
TAIEQGLEVMPVLNKIDLPQADPDRVKEEIEKIIGIDATDAVECSAKTGLGVDEVLERLV
HTIPAPTGNYEDPLQALIIDSWFDNYLGVVSLVRVRHGRVKKGDKILVKSTGKIHLVDSV
GVFNPKHTPTVDLKAGEVGFIIASIKDIHGAPVGDTLTLSSTPDVDVLPGFKRIQPQVYA
GLFPVSSDDFEDFREALQKLTLNDSSLQYTPESSDALGFGFRCGFLGMLHMEIIQERLER
EYNLDLITTAPTVIFELLLKTGETIYVDNPSKLPDLSSIEDMREPIVRANILVPQEHLGN
VITLCIEKRGVQHDMLFLGTQVQVTYDLPMNEVVLDFFDRLKSTSRGYASLDYHFDRYQS
ASLVKLDVLINGDKVDALALIVHRDNAHYKGRQLTEKMKELIPRQMFDVAIQAAIGGQIV
ARTTVKALRKNVLAKCYGGDVSRKKKLLEKQKAGKKRMKQVGNVEIPQEAFLAVLRLDS