Protein Info for Pf1N1B4_892 in Pseudomonas fluorescens FW300-N1B4

Annotation: Ribonuclease III (EC 3.1.26.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 208 PF14622: Ribonucleas_3_3" amino acids 3 to 118 (116 residues), 125 bits, see alignment E=3.2e-40 TIGR02191: ribonuclease III" amino acids 3 to 199 (197 residues), 213.3 bits, see alignment E=1.6e-67 PF00636: Ribonuclease_3" amino acids 16 to 106 (91 residues), 90 bits, see alignment E=2.4e-29 PF00035: dsrm" amino acids 134 to 199 (66 residues), 41.7 bits, see alignment E=2.2e-14

Best Hits

Swiss-Prot: 98% identical to RNC_PSEPF: Ribonuclease 3 (rnc) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03685, ribonuclease III [EC: 3.1.26.3] (inferred from 98% identity to pfo:Pfl01_0994)

MetaCyc: 55% identical to RNase III (Escherichia coli K-12 substr. MG1655)
Ribonuclease III. [EC: 3.1.26.3]

Predicted SEED Role

"Ribonuclease III (EC 3.1.26.3)" in subsystem RNA processing and degradation, bacterial or Two cell division clusters relating to chromosome partitioning (EC 3.1.26.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.3

Use Curated BLAST to search for 3.1.26.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MWA1 at UniProt or InterPro

Protein Sequence (208 amino acids)

>Pf1N1B4_892 Ribonuclease III (EC 3.1.26.3) (Pseudomonas fluorescens FW300-N1B4)
MVLALTHRSFAGRNNERLEFLGDAILNFVAGEALFDRFPLAREGQLSRLRARLVKGETLA
VLARGFDLGEYLRLGSGELKSGGFRRESILADALEALIGAIYLDAGMDMARERVLAWLAG
EFEGLTLVDTNKDPKTRLQEFLQSRGCELPRYEVVDIQGEPHCRTFFVECEIILLNEKSR
GQGVSRRIAEQVAAAAALIALGVENGND