Protein Info for Pf1N1B4_891 in Pseudomonas fluorescens FW300-N1B4

Annotation: GTP-binding protein Era

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00231: small GTP-binding protein domain
  • amino acids 9 to 166 (158 residues), 99.5 bits, E=1.7e-32
• TIGR00436: GTP-binding protein Era
  • amino acids 10 to 280 (271 residues), 331.3 bits, E=4.5e-103
• PF02421: FeoB_N
  • amino acids 12 to 168 (157 residues), 53.9 bits, E=7.2e-18
• PF10662: PduV-EutP
  • amino acids 12 to 170 (159 residues), 21.2 bits, E=1e-07
• PF01926: MMR_HSR1
  • amino acids 12 to 126 (115 residues), 90.4 bits, E=4e-29
• PF04548: AIG1
  • amino acids 13 to 134 (122 residues), 37.2 bits, E=9e-13
• PF00009: GTP_EFTU
  • amino acids 78 to 175 (98 residues), 28.2 bits, E=6.3e-10
• PF07650: KH_2
  • amino acids 210 to 285 (76 residues), 76.3 bits, E=6.1e-25

Best Hits

Swiss-Prot: 97% identical to ERA_PSEPF: GTPase Era (era) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03595, GTP-binding protein Era (inferred from 98% identity to pba:PSEBR_a4553)

MetaCyc: 59% identical to 30S ribosomal subunit maturation GTPase Era (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein Era" in subsystem Bacterial Cell Division or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A166MW94 at UniProt or InterPro

Protein Sequence (300 amino acids)

>Pf1N1B4_891 GTP-binding protein Era (Pseudomonas fluorescens FW300-N1B4)
MTDSTATRCGYVAIVGRPNVGKSTLLNHILGQKLAITSRKPQTTRHNMLGIKTEGAVQAI
YVDTPGMHKGGEKALNRYMNKTASAALKDVDVVIFVVDRTKWTDEDQMVLERVQYVTGPL
IVALNKTDRIEDKSELMPHLSWLQEQLPNAQIMPISAQHGHNLDALERVIAEHLPENDHF
FPEDQITDRSSRFLAAELVREKIMRQLGAELPYQITVEIEEFKQQGKTLHIHALILVERD
GQKKIIIGDKGERIKRIGTEARKDMELLFDSKIMLNLWVKVKGGWSDDERALRSLGYGDL