Protein Info for Pf1N1B4_890 in Pseudomonas fluorescens FW300-N1B4

Annotation: DNA recombination and repair protein RecO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 229 PF11967: RecO_N" amino acids 8 to 73 (66 residues), 44.8 bits, see alignment E=9.8e-16 TIGR00613: DNA repair protein RecO" amino acids 12 to 148 (137 residues), 59.9 bits, see alignment E=1.3e-20 PF02565: RecO_C" amino acids 83 to 224 (142 residues), 69.1 bits, see alignment E=3.9e-23

Best Hits

Swiss-Prot: 93% identical to RECO_PSEF5: DNA repair protein RecO (recO) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K03584, DNA repair protein RecO (recombination protein O) (inferred from 94% identity to pba:PSEBR_a4552)

Predicted SEED Role

"DNA recombination and repair protein RecO" in subsystem DNA repair, bacterial RecFOR pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MW84 at UniProt or InterPro

Protein Sequence (229 amino acids)

>Pf1N1B4_890 DNA recombination and repair protein RecO (Pseudomonas fluorescens FW300-N1B4)
MSPTQPIAQPAYVLHSRAYRESSALVDFLTPQGRLRAVLRSARGKAGTLARPFVPLEVEF
RGRGELKNVGRMESAGVSTWLNGEALFSGLYLNELLIRLLPAEDPHPAVFDHYAATLLAL
AEGRPLEPLLRSFEWRLLDDLGYGFALNTDIHGDPIAPDGLYRLQVDAGLERVYLLQPGL
FNGTELLAMADADWSAPGALSAAKRLMRQALAVHLGGRPLVSRELFRKP