Protein Info for Pf1N1B4_887 in Pseudomonas fluorescens FW300-N1B4
Annotation: Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to PUR4_HAHCH: Phosphoribosylformylglycinamidine synthase (purL) from Hahella chejuensis (strain KCTC 2396)
MetaCyc: 65% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]
Predicted SEED Role
"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (20/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
- superpathway of 5-aminoimidazole ribonucleotide biosynthesis (5/6 steps found)
- 5-aminoimidazole ribonucleotide biosynthesis I (4/5 steps found)
- 5-aminoimidazole ribonucleotide biosynthesis II (4/5 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.5.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166MW62 at UniProt or InterPro
Protein Sequence (1298 amino acids)
>Pf1N1B4_887 Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3) (Pseudomonas fluorescens FW300-N1B4) MLILRGAPALSAFRHSKLLEQLSQKVPAVSGLYAEFAHFAEVTGVLTGDEQQVLARLLKY GPSVPVQEPTGRLFLVLPRFGTISPWSSKASDIARNCGLTKVQRLERGIAFYVAGQFSDT EAQLIADALHDRMTQIVLDNLEQAAGLFSHAEPKPLTAIDVLGGGRAALEKANTELGLAL AEDEIDYLVAAFVGLKRNPHDIELMMFAQANSEHCRHKIFNASWDIDGQSQEKSLFGMIK NTYQMHSEGVLSAYKDNASVIVGSVAGRFFPDPETRQYGAVQEPVHILMKVETHNHPTAI APFPGASTGSGGEIRDEGATGRGAKPKAGLTGFTVSNLQIPGFEQPWEVPYGKPERIVTA LDIMIEGPLGGAAFNNEFGRPALTGYFRTFEQSITTPRGDEVRGYHKPIMLAGGMGNIRA EHVQKGEITVGSKLIVLGGPAMLIGLGGGAASSMATGTSSADLDFASVQRENPEMERRCQ EVIDRCWQLGDKNPISFIHDVGAGGLSNAFPELVNDGNRGGRFELRNIPNDEPGMAPHEI WSNESQERYVLAVGPADFERFQAICERERCPFAVVGEATAEPQLTVTDSHFGNSPVDMPL EVLLGKAPRMHRSAVRENELGDDFDPSTLEIADCVERVLHHPAVASKSFLITIGDRTITG LVARDQMVGPWQVPVADVAVTATSFDVYTGEAMAMGERTPLALLDAPASGRMAIGETLTN IAASRIAKISDIKLSANWMSAAGHPGEDARLYDTVKAVGMELCPDLGITIPVGKDSMSMA TRWNDEGVDKTVTSPMSLIVTGFAPVADIRQTMTPQLRMDKGTTDLILIDLGRGQNRMGA SILAQVHGKLGKQAPDVDDAEDLKAFFAVIQGLNADGHLLAYHDRSDGGLLTSVVEMAFA GHCGLSLNLDGLAETSADIAAILFNEELGAVIQVRQDATPDILAQFSAAGLGDCVSVIGQ PMNNGEISITFNGDTVFEGQRRLLQRQWAETSYQIQRLRDNADCAEQEFDVLLEEDNPGL SVKLSYDVNQDVAAPYIKKGIRPQVAVLREQGVNGQVEMAAAFDRAGFNAIDVHMSDILA GRVDLNEFKGLVACGGFSYGDVLGAGEGWAKSALFNSRARDAFQGFFERNDSFTLGVCNG CQMMSNLHELIPGSEFWPHFVRNRSEQFEARVAMVQIQESNSIFLQGMAGSRMPIAIAHG EGHAEFASEEALLEADLSGCVAMRFVDNHGKVTENYPANPNGSPRGITGLTSRDGRVTIM MPHPERVFRAVQNSWRSEDWNEDAPWMRMFRNARVWVN