Protein Info for Pf1N1B4_875 in Pseudomonas fluorescens FW300-N1B4
Annotation: Glutamate synthase [NADPH] small chain (EC 1.4.1.13)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_1017)Predicted SEED Role
"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13
Use Curated BLAST to search for 1.4.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166MVX7 at UniProt or InterPro
Protein Sequence (267 amino acids)
>Pf1N1B4_875 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) (Pseudomonas fluorescens FW300-N1B4) LSPSLLTTLAAACLYAAATLYQGTRLATGAKANKRLLVTLGVLAVLAHSVSLITYLLTPI GLALDFFSAASLIAAAVIALTLLACSRIPVENLLLLLFPLGAATVVLAQFAPAGTVQIID EAPGILAHILLSILAYGMFTIAVFQSLLLLVQDHQLKHKHPSGLIKNFPPLQTMESLLFG FLWAGWSLLSLSLISGWLFVENLFAQHLVHKTLLACLAWIVFSVLLWGRNRLGWRGHKAI RWTLAGFCLLMLAYFGSKLVREYILHI