Protein Info for Pf1N1B4_87 in Pseudomonas fluorescens FW300-N1B4

Annotation: DNA polymerase II (EC 2.7.7.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 795 PF21474: DNApolII_N" amino acids 15 to 50 (36 residues), 79.9 bits, see alignment (E = 1.3e-26) PF03104: DNA_pol_B_exo1" amino acids 80 to 306 (227 residues), 35.2 bits, see alignment E=1.2e-12 PF00136: DNA_pol_B" amino acids 388 to 748 (361 residues), 131.9 bits, see alignment E=4.5e-42

Best Hits

Swiss-Prot: 68% identical to DPO2_ECOLI: DNA polymerase II (polB) from Escherichia coli (strain K12)

KEGG orthology group: K02336, DNA polymerase II [EC: 2.7.7.7] (inferred from 73% identity to bxe:Bxe_B0929)

MetaCyc: 68% identical to DNA polymerase II (Escherichia coli K-12 substr. MG1655)
3.1.11.-; DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"DNA polymerase II (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MF35 at UniProt or InterPro

Protein Sequence (795 amino acids)

>Pf1N1B4_87 DNA polymerase II (EC 2.7.7.7) (Pseudomonas fluorescens FW300-N1B4)
LNKIVEVAVVDLQQGFVLTRHWRDTPAGTEVEFWLATDAGPRRVRLPHQPSVAFIPAAQR
EQAEGMLHGEKNVELKPLALLDFEHRPVLGLYCQQHGQLMRLETALRRSSVDVFEADVRP
PERYMMERFITAPVLFGGTPSAEGLLLDAQMKPDPGYRPNLRLVSLDIETTAQGELYSIA
LEGCGERQVYMLGPPNGDDRGVDFQLEYCDSRTLLLKKLNEWFARHDPDAIIGWNVVQFD
LRVLHEHARRLAVPLKLGRGGEEMHWREHGSGNHYFASAAGRLIIDGIESLRSATWSFPS
FSLENVAQTLLGEGKSIDNPYQRMDEINRMFAEDKPALAKYNLKDCELVTRIFAKTELLT
FLLERASVTGLPADRSGGSVAAFTHLYMPLMHRQGFVAPNLGGKPPQASPGGFVMDSQPG
LYESVLVLDYKSLYPSIIRTFLIDPVGLIEGLKHPDDSESVPGFRGARFSRTRHCLPAIV
ARVAEGRETAKREHNVPLSQALKIIMNAFYGVLGSSGCRFFDTRLASSITLRGHEIMLRT
RQLIEAQGHAVIYGDTDSTFVWLRRAHGQAEAAQIGHALVDHVNQWWREHVKQEYGLESA
LELQFETHYKRFLMPTIRGAEEGSKKRYAGLVTRADGTDEMVYKGLETVRTDWSPLARQF
QQELYLRIFNRKPYQDYVRDYVRKTLAGEFDERLIYRKRLRRTLDDYQRNVPPHVRAARI
ADDYNDQQGRPRQYQNGGWISYVITVAGPEPLEIRSAPIDYDHYVTRQLQPVADAILPFV
DDDFSTLIGGQLGLF