Protein Info for Pf1N1B4_851 in Pseudomonas fluorescens FW300-N1B4

Annotation: Outer membrane protein YfgL, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and NlpB)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR03300: outer membrane assembly lipoprotein YfgL" amino acids 8 to 380 (373 residues), 445.1 bits, see alignment E=7.9e-138 PF13360: PQQ_2" amino acids 62 to 112 (51 residues), 22.9 bits, see alignment 9.1e-09 amino acids 79 to 309 (231 residues), 180.7 bits, see alignment E=5.7e-57 amino acids 292 to 381 (90 residues), 22.2 bits, see alignment E=1.5e-08 PF01011: PQQ" amino acids 70 to 104 (35 residues), 22.2 bits, see alignment 1.3e-08 amino acids 111 to 146 (36 residues), 29.3 bits, see alignment 7.8e-11 amino acids 246 to 275 (30 residues), 22.6 bits, see alignment (E = 9.7e-09) PF13570: PQQ_3" amino acids 89 to 128 (40 residues), 22.1 bits, see alignment 2.5e-08 amino acids 129 to 168 (40 residues), 33.5 bits, see alignment 6.3e-12 amino acids 237 to 264 (28 residues), 21.7 bits, see alignment (E = 3.4e-08)

Best Hits

Swiss-Prot: 72% identical to BAMB_PSEAE: Outer membrane protein assembly factor BamB (bamB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 97% identity to pba:PSEBR_a939)

Predicted SEED Role

"Outer membrane protein YfgL, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and NlpB)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MVI8 at UniProt or InterPro

Protein Sequence (383 amino acids)

>Pf1N1B4_851 Outer membrane protein YfgL, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and NlpB) (Pseudomonas fluorescens FW300-N1B4)
MRDVIRWKHAALLALAILAAGCSSNSKKELPPAELTDFKEEVVLQKQWSRSIGDGQGETY
NMLVPAIDGDTIYAADVTGVVMAMDRSNGDVKWKKDLELPVSGAVGVGYGLVMIGTLKGE
IVALDASSGEEKWRARVTSEVLAPPATNGDVVVVQTQDDRLIGLDAATGNQRWLYDSTPA
VLTLRGTSAPIVTNRLAVAGLSTGKVVALDVSNGVPVWEQRVAIPQGRSELERVVDIDGG
LLLSGGTLYVASYQGRVAALDLESGRQLWQRDASSYAGVAQGFGSVYVSLSAGTVEGVDE
RSTTALWSNDSLARRQLSAPEVFSSYVAVGDMEGYLHLLSQVDGRFVGRERIDSDGLRAR
PLVVGDTIYLYGNSGKLEALTIK