Protein Info for Pf1N1B4_847 in Pseudomonas fluorescens FW300-N1B4

Annotation: 2-isopropylmalate synthase (EC 2.3.3.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 TIGR00970: 2-isopropylmalate synthase" amino acids 7 to 550 (544 residues), 792.4 bits, see alignment E=1.2e-242 PF00682: HMGL-like" amino acids 33 to 313 (281 residues), 255.2 bits, see alignment E=7.8e-80 PF08502: LeuA_dimer" amino acids 422 to 550 (129 residues), 96.4 bits, see alignment E=1.3e-31

Best Hits

Swiss-Prot: 97% identical to LEU1_PSEPF: 2-isopropylmalate synthase (leuA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 97% identity to pfo:Pfl01_4594)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162ARN6 at UniProt or InterPro

Protein Sequence (559 amino acids)

>Pf1N1B4_847 2-isopropylmalate synthase (EC 2.3.3.13) (Pseudomonas fluorescens FW300-N1B4)
MSMLKDPSSKYRAFPTIDIPDRTWPSKTITAAPIWCSSDLRDGNQSLIEPMDAVKKLRFW
KTLVQVGVKEIEASFPAASQTDFDFVRTLIEDGHIPDDTTIQVLTQGREDLIARTFESLR
GAKKAIVHLYNATSPSFRRIVFNQDKDGIKAIAVNAAKLFVKYAAQQPDTEWTFEYSPET
FSATELEFAKEVCDAVIEVWNPTPEHKVILNLPATVECATPNIYADQIEWFGRHINRRDS
VIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNLYTQGVDPE
LDFSDIDGVRKVVEECNQIQVHPRHPYVGDLVHTAFSGSHQDAIRKGFAQQQPDALWEVP
YLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGINLPRRMQIEFSQVVQRETDRLG
LEMTAQQIHALLHSEYLQANTPYALVSHRLQEENGHSAVEVEVSSKGQGETNLHWRGKGN
GALEALVAGLPIPVEIMDYNEHAIGAGTNAKAAAYIELRVNGERAVHGVGIDENITTASF
KALFSALNRSLSQPEAKAA