Protein Info for Pf1N1B4_844 in Pseudomonas fluorescens FW300-N1B4

Annotation: Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 PF13742: tRNA_anti_2" amino acids 25 to 117 (93 residues), 102.5 bits, see alignment E=1.8e-33 TIGR00237: exodeoxyribonuclease VII, large subunit" amino acids 26 to 367 (342 residues), 428.7 bits, see alignment E=1.2e-132 PF01336: tRNA_anti-codon" amino acids 45 to 119 (75 residues), 50.5 bits, see alignment E=2.4e-17 PF02601: Exonuc_VII_L" amino acids 141 to 452 (312 residues), 371.6 bits, see alignment E=6e-115

Best Hits

Swiss-Prot: 97% identical to EX7L_PSEPF: Exodeoxyribonuclease 7 large subunit (xseA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03601, exodeoxyribonuclease VII large subunit [EC: 3.1.11.6] (inferred from 97% identity to pfo:Pfl01_4592)

MetaCyc: 45% identical to exodeoxyribonuclease VII subunit XseA (Escherichia coli K-12 substr. MG1655)
Exodeoxyribonuclease VII. [EC: 3.1.11.6]

Predicted SEED Role

"Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)" in subsystem DNA repair, bacterial (EC 3.1.11.6)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.6

Use Curated BLAST to search for 3.1.11.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MVD5 at UniProt or InterPro

Protein Sequence (468 amino acids)

>Pf1N1B4_844 Exodeoxyribonuclease VII large subunit (EC 3.1.11.6) (Pseudomonas fluorescens FW300-N1B4)
LPNAFKLRPMIKDPFARLGLDREVLTVSQLNGRARVLLEDVFSNIWVEGEISNLARPASG
HVYFTLKDSGAQVRCALFRQNAARVRQALKDGLAVKVRGKVSLFEGRGDYQLILDTVEPA
GDGALRLAFDALKEKLSAEGLFSAERKVPLPAHPQRIGIISSPTGAVIRDIISVFRRRAP
QIQLTLIPTAVQGREATAQIVRALKLADARGFDALILARGGGSLEDLWCFNEEAVARAVD
ACVTPIVSAVGHETDVSISDFVADVRAPTPSAAAELLAPDSSDLVRRVESLHRRLVMRMR
DRLMRDRLRLEGISRRLRHPGERLRQQAQRLDDLDMRMRRAFERSLNTRRERLIRLETRL
AGQHPGRQLAMLRQRLDSLAERLPRAMREGLKSRRLQLQSQMQTLHVVSPLATLARGYSI
LLDERGNAIRSAAQTHTGQRLKAKLGEGELQVRVEDNHLTPVTLSLLD