Protein Info for Pf1N1B4_835 in Pseudomonas fluorescens FW300-N1B4

Annotation: Trehalose-6-phosphate hydrolase (EC 3.2.1.93)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 549 TIGR02403: alpha,alpha-phosphotrehalase" amino acids 4 to 545 (542 residues), 854.7 bits, see alignment E=1.5e-261 PF00128: Alpha-amylase" amino acids 26 to 363 (338 residues), 406.7 bits, see alignment E=1.6e-125 PF11941: DUF3459" amino acids 450 to 512 (63 residues), 23.6 bits, see alignment E=8.3e-09 PF16657: Malt_amylase_C" amino acids 467 to 542 (76 residues), 33.3 bits, see alignment E=6.4e-12

Best Hits

Swiss-Prot: 58% identical to TREC_BACSU: Trehalose-6-phosphate hydrolase (treA) from Bacillus subtilis (strain 168)

KEGG orthology group: K01226, trehalose-6-phosphate hydrolase [EC: 3.2.1.93] (inferred from 88% identity to pfs:PFLU5039)

MetaCyc: 58% identical to trehalose-6-phosphate hydrolase (Bacillus subtilis)
Alpha,alpha-phosphotrehalase. [EC: 3.2.1.93]

Predicted SEED Role

"Trehalose-6-phosphate hydrolase (EC 3.2.1.93)" in subsystem Trehalose Uptake and Utilization (EC 3.2.1.93)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.93

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MV69 at UniProt or InterPro

Protein Sequence (549 amino acids)

>Pf1N1B4_835 Trehalose-6-phosphate hydrolase (EC 3.2.1.93) (Pseudomonas fluorescens FW300-N1B4)
MQDWQRSVIYQIYPKSFHSHAGNPTGDLLGVVAKLDYLHWLGVDCLWITPFLRSPQRDNG
YDISDYYAIDPSYGTMADCELLIAEAGKRGIKLMLDIVVNHTSIEHTWFQQARSSLDNPY
RDFYIWRDQPNNWESKFGGSAWEYEAQTGQYYLHLFDHTQADLNWDNPQVRAEVFKMMRF
WRDKGVGGFRLDVINLISKPADFPEDNSDGRRFYTDGPNVHEYLQQMHREVFEGHDLINV
GEMSSTSLEHCIRYSRPESKELSMTFNFHHLKVDYPNLQKWVRADFDFLALKRILSDWQT
GMQAGGGWNALFWCNHDQPRVVSRFGHDGEHRVVSAKMLGTALHFLQGTPFVYQGEELGM
TNPGFDHIDQYRDVETLNIFRLKREAGSSDVDNMAAIMQKSRDNGRTPMHWNTEPNAGFS
AVEPWIGVPANAAQINVAHQLDDPDSVLHHYRQLIALRRSETLMSDGVYRQLLPEHTQIW
AYVREGQGERLLVLNNFYGTPCEVELPPEVINESMVQSLVISNYPDCPPRNRQVFLRPYE
SFVLRLTDH