Protein Info for Pf1N1B4_822 in Pseudomonas fluorescens FW300-N1B4

Annotation: ATP-dependent protease La (EC 3.4.21.53) Type I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 PF02190: LON_substr_bdg" amino acids 41 to 236 (196 residues), 83.7 bits, see alignment E=4.5e-27 TIGR00763: endopeptidase La" amino acids 42 to 805 (764 residues), 908.5 bits, see alignment E=2.3e-277 PF07728: AAA_5" amino acids 387 to 520 (134 residues), 32.4 bits, see alignment E=2.3e-11 PF00004: AAA" amino acids 387 to 526 (140 residues), 89.3 bits, see alignment E=7.4e-29 PF05362: Lon_C" amino acids 607 to 803 (197 residues), 251.9 bits, see alignment E=1.1e-78 PF13541: ChlI" amino acids 653 to 774 (122 residues), 30.9 bits, see alignment E=5.6e-11

Best Hits

Swiss-Prot: 59% identical to LON2_HYDCU: Lon protease 2 (lon2) from Hydrogenovibrio crunogenus (strain XCL-2)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 95% identity to pfo:Pfl01_4579)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161Z4U7 at UniProt or InterPro

Protein Sequence (805 amino acids)

>Pf1N1B4_822 ATP-dependent protease La (EC 3.4.21.53) Type I (Pseudomonas fluorescens FW300-N1B4)
MTDQQEFPEDTSEYTETDNAEHHSTGKGLALPGQNLPDKVYIIPIHNRPFFPAQVLPVIV
NEEPWAETLELVSQSDHHSLALFFMDTPQEDPRHFDTKALPEYGTLVKVHHASRENGKLQ
FVAQGLSRVRIKTWLKHHRPPYLVEVEYPHQPTEPTDEVKAYGMALINAIKELLPLNPLY
SEELKNYLNRFSPNDPSPLTDFAAALTSATGSELQEVLDCVPMLKRMEKVLPMLRKEVEV
ARLQKEISAEVNRKIGEHQREFFLKEQLKVIQQELGLTKDDRSADIEQFEQRLTGKVLPP
QAQKRIEEEMNKLSILETGSPEYAVTRNYLDWATSVPWGVYGEDKLDLKHARKVLDKHHA
GLDDIKDRILEFLAVGAYKGEISGSIVLLVGPPGVGKTSVGKSIAESLGRPFYRFSVGGM
RDEAEIKGHRRTYIGAQPGKLVHALKDVEVMNPVIMLDEIDKMGQSYQGDPASALLETLD
PEQNVEFLDHYLDLRLDLSKVLFVCTANTLDSIPGPLLDRMEVIRLSGYITEEKVAIAKR
HLWPKQLEKAGVSKGSLSISDNALKALIDGYAREAGVRQLEKNLGKLVRKAVMKLLDEPK
AVIKLGPKDLEASLGRPVFRNEQVLSGIGVITGLAWTSMGGATLPIEATRIHTLNRGFKL
TGQLGEVMKESAEIAYSYVSSHLKQFGGDPTFFDEAFVHLHVPEGATPKDGPSAGVTMAS
ALLSLARNQPPKKGVAMTGELTLTGHVLPIGGVREKVIAARRQKIFELILPEPNRGSFEE
LPDYLKEGITVHFAKRFADVAKILF