Protein Info for Pf1N1B4_821 in Pseudomonas fluorescens FW300-N1B4

Annotation: Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 802 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 41 to 59 (19 residues), see Phobius details amino acids 66 to 82 (17 residues), see Phobius details amino acids 88 to 110 (23 residues), see Phobius details amino acids 122 to 143 (22 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 16 to 800 (785 residues), 1171.3 bits, see alignment E=0 PF01011: PQQ" amino acids 230 to 258 (29 residues), 24.9 bits, see alignment (E = 1.9e-09) amino acids 307 to 340 (34 residues), 27 bits, see alignment (E = 4.1e-10) amino acids 461 to 486 (26 residues), 22 bits, see alignment (E = 1.5e-08) amino acids 676 to 709 (34 residues), 24.8 bits, see alignment (E = 2e-09) amino acids 732 to 766 (35 residues), 22.5 bits, see alignment (E = 1e-08) PF13360: PQQ_2" amino acids 239 to 526 (288 residues), 41 bits, see alignment E=2.7e-14 amino acids 682 to 769 (88 residues), 22.3 bits, see alignment E=1.4e-08

Best Hits

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 89% identity to pba:PSEBR_a4532)

Predicted SEED Role

"Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway or Respiratory dehydrogenases 1 (EC 1.1.5.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MUW2 at UniProt or InterPro

Protein Sequence (802 amino acids)

>Pf1N1B4_821 Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2) (Pseudomonas fluorescens FW300-N1B4)
MSADGAFSRSRLLPSLLGIVLLLMGLALLAGGIKLSMLGGSLYYLLAGIGLMLTGGLLLA
DRYAALSLYAVVLFASTVWALWEVGLDWWQLVPRLALLFALGIVMLLPWFRRPLRTGEFN
PVGTGVLSAAVVIAGIAALASLFTNPGEIKGELDRDAVPGMSNTAPAMPDGDWNSYGRSA
HGDRYSPLAQITPQNVNKLVPAWTYRTGDLPGPNDPGETTAENTPLKANGMLYVCTPHSQ
VIALDPDTGKELWRFDPKLSTQNAANFKGWAHMTCRGVTYHDDAAYASEQSPTGTANAPV
ASVCPRRIFLPTADTRLIALNADTGKMCEDFGNGGQVDLTTNIGEFTAGGYYSTSPPAVT
KDLVVIGGHVTDNVSIDEPSGVIRAFDVHTGKLVWNWDSGNPDDTTPIAEGKVYTRNSPN
MWSIFSVDEKLGMIYLPMGNQTPDQFGGARTPQSELHAAGLTALDIATGKVRWHFQFTHH
DLWDMDVGGQPTLMDLKTADGVKPAVLASTKQGSIYVLDRSNGQPIVPIKEIPVPQGAVE
GDYTSPTQPMSDLNFVPPTLKERDMWGVTPFDQMLCRIDFKSLRYDGMFTPPSLQGSIVY
PGNFGVFDWGGMSVDPVRQIAFVNPSYMAFKSKLIPAAEIAKQGPRVSETEGVQPNKGAP
YGVILEALLSPMGLPCQAPAWGYVAAVDLTTNKTIWMHKNGTVRDSSPVPIPLSMGVPSL
GGAFTTASGVAFLSGTLDQYLRAYDVKNGKQLWEGRLPAGAQTTPMTYTGKDGKQYVLVV
AGGHGSLGTKQGDYVIAYKLSE