Protein Info for Pf1N1B4_798 in Pseudomonas fluorescens FW300-N1B4

Annotation: Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 transmembrane" amino acids 228 to 249 (22 residues), see Phobius details amino acids 261 to 286 (26 residues), see Phobius details PF00535: Glycos_transf_2" amino acids 4 to 165 (162 residues), 110.3 bits, see alignment E=5.2e-36

Best Hits

Swiss-Prot: 66% identical to GTRB_BPSF5: Bactoprenol glucosyl transferase (gtrB) from Shigella phage SfV

KEGG orthology group: None (inferred from 66% identity to ppw:PputW619_0863)

MetaCyc: 64% identical to CPS-53 (KpLE1) prophage; bactoprenol glucosyl transferase (Escherichia coli K-12 substr. MG1655)
Phosphopolyprenol glucosyltransferase. [EC: 2.4.1.78]

Predicted SEED Role

"Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-)" in subsystem Lipid A-Ara4N pathway ( Polymyxin resistance ) (EC 2.4.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.-.-

Use Curated BLAST to search for 2.4.-.- or 2.4.1.78

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162ARK0 at UniProt or InterPro

Protein Sequence (313 amino acids)

>Pf1N1B4_798 Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-) (Pseudomonas fluorescens FW300-N1B4)
VKVSLIVPVFNEEQAISLFHQAVRRELKLDPCEVEIVFINDGSTDRTAEQAKALAQVDEQ
VLLINFSRNFGKEPALFAGLEYATGDAVIPMDVDLQDPISVIPQLITEWQKGADVVLAKR
RNRDSDSYLKRHSASLFYHLLNRISYTRIEENVGDFRLMDRKVVNVIRALPEHQLFMKGV
LSWAGFTTAVVEYERARRVAGRSKFNGWKLWNLALEGITSFSTVPLRLWTYIGGGISIFA
VLYAVYMVLDKIFFGNNVPGYPSLMTAILFLGGVQLIGIGILGEYVGRIYIEAKHRPRYV
VKDVIGGKDRTGL