Protein Info for Pf1N1B4_73 in Pseudomonas fluorescens FW300-N1B4

Annotation: Beta-lactamase (EC 3.5.2.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF00144: Beta-lactamase" amino acids 41 to 388 (348 residues), 286.6 bits, see alignment E=1.4e-89

Best Hits

Swiss-Prot: 67% identical to AMPC_PSEFL: Beta-lactamase (ampC) from Pseudomonas fluorescens

KEGG orthology group: K01467, beta-lactamase [EC: 3.5.2.6] (inferred from 70% identity to pfo:Pfl01_3723)

MetaCyc: 45% identical to beta-lactamase (Escherichia coli K-12 substr. MG1655)
Beta-lactamase. [EC: 3.5.2.6]

Predicted SEED Role

"Beta-lactamase (EC 3.5.2.6)" in subsystem Beta-lactamase or Tn552 (EC 3.5.2.6)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MES2 at UniProt or InterPro

Protein Sequence (390 amino acids)

>Pf1N1B4_73 Beta-lactamase (EC 3.5.2.6) (Pseudomonas fluorescens FW300-N1B4)
MENDSIMRIKNQRKLASYSAFALLLGVSHSFADDRIEAIVKAAVEPVMQQQQIPGVAVAI
TVNGQPHYFNYGVASKENGKAVTEDTLFEIGSVSKTFTATLAAYAHATGKLALSDNASQV
LPTLRGSAFDNISVLQLGTYTAGGLPLQFPGDADHQDKMLGYFKQWKPAYAAGTHRLYSN
PSIGLFGYLAAHSMGAPFDELMEKTLLPKLGLKHSYLKVPQDQMALYAQGYNKDDKPVRV
EPGALDSEAYGVKTSASDLLRYVEANMNPTSLEKPLQQAIATTHTGYYTVGEMTQGLGWE
FYNYPVTLDTLLAGNSSQMAFEAHEVQPLNPPQPPHDTVLINKTGSTGGFGAYVAFVPAK
DIGIVILANKNYPNPERVKIAYEIMSALAE