Protein Info for Pf1N1B4_720 in Pseudomonas fluorescens FW300-N1B4

Annotation: RND multidrug efflux transporter; Acriflavin resistance protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1008 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 335 to 353 (19 residues), see Phobius details amino acids 359 to 379 (21 residues), see Phobius details amino acids 385 to 409 (25 residues), see Phobius details amino acids 430 to 451 (22 residues), see Phobius details amino acids 463 to 489 (27 residues), see Phobius details amino acids 521 to 542 (22 residues), see Phobius details amino acids 845 to 865 (21 residues), see Phobius details amino acids 872 to 892 (21 residues), see Phobius details amino acids 899 to 922 (24 residues), see Phobius details amino acids 948 to 970 (23 residues), see Phobius details amino acids 978 to 1002 (25 residues), see Phobius details PF00873: ACR_tran" amino acids 6 to 1002 (997 residues), 929 bits, see alignment E=4.9e-283 PF03176: MMPL" amino acids 298 to 490 (193 residues), 29.9 bits, see alignment E=4.3e-11 amino acids 811 to 1003 (193 residues), 31.1 bits, see alignment E=1.9e-11

Best Hits

Swiss-Prot: 45% identical to Y895_HAEIN: Uncharacterized transporter HI_0895 (HI_0895) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: None (inferred from 94% identity to pfo:Pfl01_4493)

Predicted SEED Role

"RND multidrug efflux transporter; Acriflavin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162ARE4 at UniProt or InterPro

Protein Sequence (1008 amino acids)

>Pf1N1B4_720 RND multidrug efflux transporter; Acriflavin resistance protein (Pseudomonas fluorescens FW300-N1B4)
MAFTDPFIRRPVLATVVSLLIVLLGFQAWSKLPLRQYPQMENALITVTTAYPGANAETIQ
GYITQPMQQSLASAEGIDYMTSVSRQNFSVISIYARIGANSDRLFTELLAKANEVKNQLP
QDAEDPVLSKEAADASALMYISFFSDELSNPQITDYLSRVIQPKLATLPGMAEAEILGNQ
VFAMRLWLDPVKLAGYGLSASDVTNAVRQYNFLSAAGEVKGEYVVTSINANTELKSAEAF
GAIPLKVDGDSRVLLRDVARVEMGAENYNTISSFGGTPSVYIGIKATPGANPLDVIREVR
KIMPELEAQLPPNLKGEIAYDATLFIQASIDEVVKTLFEAVLIVIVVVFLFLGALRSVVI
PVVTIPLSMIGVMFFMQMMGYSINLLTLLAMVLAIGLVVDDAIVVVENIHRHIEEGKTPL
DAAIEGAREIAMPVVSMTITLAAVYAPIGFLTGLTGALFKEFALTLAGAVVISGVVALTL
SPMMCALLLRHDENPTGLAHRLDVIFEGLKRRYQSLLHGTLNTRPVVLVFAVIVLCLIPV
LLKFTKSELAPDEDQGVIFMMASAPQTANLDYLNTYTDEFLTIFKEFPEYYSSFQINGFN
GVQSGIGGFLLKPWNERDRTQMQILPEVQSKLESIPGLQIFGFNLPSLPGTGEGLPFQFV
INSANTYESLLEVTDRVKKRAMESGKFAFVDVDLAFDKPEVVVDIDRAKAAQMGVSMQAL
GGTLATLLGEAEINRFTIEGRSYKVIAQVERPFRDNPDWLNHYYVKNTKGELLPLSTLIT
VTDRARPRQLNQFQQLNSAILSGVPLVSMGEAIDTVRQIAREEAPAGYAVDYAGASRQYV
QEGSALWVTFALALAIIFLVLAAQFESFRDPLVILVTVPLSICGALIPLFLGWSSMNIYT
QVGLVTLIGLISKHGILIVEFANQLRKEKGLSPREAVEEAAAIRLRPVLMTTAAMVFGMV
PLIIATGAGAVSRFDIGTVIATGMSIGTLFTLFVLPCIYTLLAKPDKP