Protein Info for Pf1N1B4_71 in Pseudomonas fluorescens FW300-N1B4
Annotation: Glucarate dehydratase (EC 4.2.1.40)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 80% identical to GUDD_PSEPU: Glucarate dehydratase (gudD) from Pseudomonas putida
KEGG orthology group: K01706, glucarate dehydratase [EC: 4.2.1.40] (inferred from 95% identity to pfo:Pfl01_3721)MetaCyc: 80% identical to D-glucarate dehydratase subunit (Pseudomonas putida)
Glucarate dehydratase. [EC: 4.2.1.40]
Predicted SEED Role
"Glucarate dehydratase (EC 4.2.1.40)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.40)
MetaCyc Pathways
- D-glucarate degradation II (3/3 steps found)
- superpathway of D-glucarate and D-galactarate degradation (4/5 steps found)
- D-glucarate degradation I (3/4 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (14/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.40
Use Curated BLAST to search for 4.2.1.40
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166MEP6 at UniProt or InterPro
Protein Sequence (447 amino acids)
>Pf1N1B4_71 Glucarate dehydratase (EC 4.2.1.40) (Pseudomonas fluorescens FW300-N1B4) MNPQEAAKAPIITSMQVVPVAGHDGMLLNLSGAHGPFFTRNVVILKDNAGHTGVGEVPGG ERIRQTLEDARALVVGSPIGTYQKILNQVRQTFADRDAGGRGLQTFDLRITIHAVTGLEA ALLDLLGQHLDVPVAALLGEGQQRDEVKMLGYLFYVGDRNETDLAYRSEPDADNDWFRVR HEKAMSADAVVRLAEAAHARYGFKDFKLKGGVLSGDEEIEAVTALAERFPDARITLDPNG AWSLKEAIRLCRDQHHVLAYAEDPCGAENSYSGREVMAEFRRATGLKTATNMIATDWREM GHAIQLQSVDIPLADPHFWTLQGSVRVAQMCHEWGLTWGSHSNNHFDISLAMFTHVAAAA PGEITAIDTHWIWQDGQRLTKAPLQIVDGCVQVPTKPGLGVELDMDQLAKAHELYKGMGL GARDDSVAMQFLIPGWKFDNKRPCLVR