Protein Info for Pf1N1B4_707 in Pseudomonas fluorescens FW300-N1B4

Annotation: Nucleoside-diphosphate-sugar epimerases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF04321: RmlD_sub_bind" amino acids 1 to 152 (152 residues), 33.9 bits, see alignment E=6.5e-12 PF05368: NmrA" amino acids 2 to 109 (108 residues), 38.2 bits, see alignment E=4.7e-13 PF01370: Epimerase" amino acids 3 to 227 (225 residues), 121.5 bits, see alignment E=1.4e-38 PF16363: GDP_Man_Dehyd" amino acids 4 to 237 (234 residues), 48.5 bits, see alignment E=3.2e-16 PF01073: 3Beta_HSD" amino acids 4 to 251 (248 residues), 110.2 bits, see alignment E=3.4e-35 PF13460: NAD_binding_10" amino acids 7 to 149 (143 residues), 58.3 bits, see alignment E=3.4e-19 PF07993: NAD_binding_4" amino acids 42 to 170 (129 residues), 54 bits, see alignment E=5.1e-18

Best Hits

KEGG orthology group: None (inferred from 94% identity to pba:PSEBR_a1254)

Predicted SEED Role

"Nucleoside-diphosphate-sugar epimerases"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MSP6 at UniProt or InterPro

Protein Sequence (330 amino acids)

>Pf1N1B4_707 Nucleoside-diphosphate-sugar epimerases (Pseudomonas fluorescens FW300-N1B4)
MKILVTGASGFIGGRFARFALEQGLDVRVNGRRAESVEHLVRRGAEFIQGDLSDPQLARD
LCSDVEAVVHCAGAVGLWGRYQDFHQGNVEVTENVVEACLKQRVRRLVHLSSPSIYFDGR
DHLGLTEEQVPKRFKHPYAATKYLAEQKVFGAQEFGLETLALRPRFVTGAGDMSIFPRLL
KMQRKGRLAIIGNGLNKVDFTSVQNLNEALLSSLLASGSALGKAYNISNGAPVPLWDVVN
YVMRQMDVPQVTRYRSYGLAYSLAALNEGVCKLWPGRPEPTLSRLGMQVMNKNFTLDISR
ARHYLDYDPKVSLWTALDEFCGWWKAQDIR