Protein Info for Pf1N1B4_7 in Pseudomonas fluorescens FW300-N1B4

Annotation: tRNA pseudouridine synthase A (EC 4.2.1.70)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 TIGR00071: tRNA pseudouridine(38-40) synthase" amino acids 18 to 255 (238 residues), 221.6 bits, see alignment E=5.5e-70 PF01416: PseudoU_synth_1" amino acids 24 to 119 (96 residues), 41.3 bits, see alignment E=9.4e-15 amino acids 159 to 260 (102 residues), 108.1 bits, see alignment E=1.6e-35

Best Hits

Swiss-Prot: 76% identical to TRUA_PSEAE: tRNA pseudouridine synthase A (truA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 97% identity to pba:PSEBR_a3861)

MetaCyc: 55% identical to tRNA pseudouridine38-40 synthase (Escherichia coli K-12 substr. MG1655)
tRNA-pseudouridine synthase I. [EC: 5.4.99.12]

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161YXJ6 at UniProt or InterPro

Protein Sequence (284 amino acids)

>Pf1N1B4_7 tRNA pseudouridine synthase A (EC 4.2.1.70) (Pseudomonas fluorescens FW300-N1B4)
LANIDNPAAEMAADGFFRIALGVEYKGSRYRGWQRQASGVLTVQETLEKALSKVADSPVS
LLCAGRTDAGVHACGQVVHFDTQVERSMKAWVMGANINLPHDVSVSWAKVMPAHFHARFK
AIARRYRYVIYNDQIRPAHLNDEITWNHRPLDVERMSEAAQYLVGTHDFSAFRAGQCQAK
SPIKELHHLRVTRHGKMIVLDIRASAFLHHMVRNIAGVLMTIGAGERPVEWMKEVLESRI
RRSGGVTAHPFGLYLVQVEYRDEFELPERYIGPHFLTGFSELDG