Protein Info for Pf1N1B4_698 in Pseudomonas fluorescens FW300-N1B4

Annotation: Rrf2-linked NADH-flavin reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 204 PF03435: Sacchrp_dh_NADP" amino acids 4 to 74 (71 residues), 27 bits, see alignment E=1e-09 PF01370: Epimerase" amino acids 4 to 80 (77 residues), 34.1 bits, see alignment E=3.9e-12 PF05368: NmrA" amino acids 4 to 94 (91 residues), 34.5 bits, see alignment E=3e-12 PF13460: NAD_binding_10" amino acids 8 to 189 (182 residues), 100.4 bits, see alignment E=2.4e-32

Best Hits

KEGG orthology group: K07118, (no description) (inferred from 84% identity to pfl:PFL_4814)

Predicted SEED Role

"Rrf2-linked NADH-flavin reductase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162ARC9 at UniProt or InterPro

Protein Sequence (204 amino acids)

>Pf1N1B4_698 Rrf2-linked NADH-flavin reductase (Pseudomonas fluorescens FW300-N1B4)
MSNIAIIGATGRAGSQLLEEALRRGHSVTAIARDTSKIAPRAGVVSKSVDVLDAAALQAA
VAGHDAVISAAHFSTIPASAVIEPVKKAGVKRLLVVGGAGSLLLPGGSRVIDSEGFPEEY
KAEASAGAAFLNTLRQEQDLDWTFLSPSAEFVEGERTGTFRIGKDDLLVSAEGRSWITFA
DFAIALLDEMQTPKYSRQRFTVGY