Protein Info for Pf1N1B4_68 in Pseudomonas fluorescens FW300-N1B4

Annotation: Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details transmembrane" amino acids 153 to 173 (21 residues), see Phobius details PF21085: CusS" amino acids 2 to 147 (146 residues), 30 bits, see alignment E=1.4e-10 TIGR01386: heavy metal sensor kinase" amino acids 3 to 446 (444 residues), 501.5 bits, see alignment E=1.2e-154 PF00672: HAMP" amino acids 171 to 223 (53 residues), 42.7 bits, see alignment 1.5e-14 PF00512: HisKA" amino acids 229 to 294 (66 residues), 56.9 bits, see alignment E=4.4e-19 PF02518: HATPase_c" amino acids 337 to 447 (111 residues), 87.9 bits, see alignment E=1.6e-28 PF14501: HATPase_c_5" amino acids 344 to 445 (102 residues), 22.7 bits, see alignment E=2e-08

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 81% identity to pba:PSEBR_a1769)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A162AQ00 at UniProt or InterPro

Protein Sequence (449 amino acids)

>Pf1N1B4_68 Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) (Pseudomonas fluorescens FW300-N1B4)
MRRLSLSNRLALLFAACTAVVSLFAGVLFSRASEAHFVELDQQLLDGKLIGLRRALDDIP
SSESETKLADELSRQADLSLRIIGSDGQRLYDSSARLPKDLPRQLGLSTVSDDGTDYRVL
NAPLFLDKPDSPQLTLLLDITHHQHFLQRMQHLIWLTVGLSALATALLGAWAARSGLRPL
RRMSAVASGVSAQSLNARLPEADMPPELAEMAHSFNAMLGRLDDSFQRLSAFSADIAHEL
RTPLSNLLTHTQVTLTRPRPIEDYREALHSNLEELQWMAQLVNDMLYLAKADHGLLMPKR
EALELAEEADVLLEFFAPLAEDAQVKLSRDGSARMQGDRSMLRRALSNLLDNALRFTPGG
GEVRVKIIEQPTGLSLTVENSGEVISEDLLPRLFDRFYRADPARREGSSEHAGLGLAITQ
SIIRAHGGQIRCESDKGWTRFVIELPKGD