Protein Info for Pf1N1B4_652 in Pseudomonas fluorescens FW300-N1B4

Annotation: Sensory box histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 678 transmembrane" amino acids 16 to 42 (27 residues), see Phobius details amino acids 155 to 179 (25 residues), see Phobius details PF00672: HAMP" amino acids 185 to 234 (50 residues), 35.2 bits, see alignment 3.1e-12 TIGR00229: PAS domain S-box protein" amino acids 288 to 403 (116 residues), 27.5 bits, see alignment E=1.4e-10 PF00989: PAS" amino acids 289 to 394 (106 residues), 46.3 bits, see alignment E=1e-15 PF08448: PAS_4" amino acids 294 to 397 (104 residues), 38.6 bits, see alignment E=2.9e-13 PF00512: HisKA" amino acids 415 to 499 (85 residues), 28.1 bits, see alignment E=4.3e-10 PF02518: HATPase_c" amino acids 552 to 664 (113 residues), 84.9 bits, see alignment E=1.4e-27

Best Hits

KEGG orthology group: K00936, [EC: 2.7.3.-] (inferred from 94% identity to pfo:Pfl01_4423)

Predicted SEED Role

"Sensory box histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QH84 at UniProt or InterPro

Protein Sequence (678 amino acids)

>Pf1N1B4_652 Sensory box histidine kinase (Pseudomonas fluorescens FW300-N1B4)
MPLRQRLENLPVGQKLLAALLVLLTTVLLVANLTFISAAYYISQESMAPQALQTIGRLVS
NPSLISDALKSPQTAERLLNELNSYTPLRAAALYDGKGVLLAQLQQGDELHLPEHFRHLE
AWQAMEFRSNQVITLPRPGTAPGHLLLVASSELPMAFYTGTLTASLGILIFSVLLWLVIA
RQIKRLITRPIHQLEELSRQVTREENYALRASRGNHDEIGSLAEAFNTMLSRIEAREQQL
KRARDDSQAAYDQAQGLAEETRHTNRKLELEVQVRSKIEKKLTGFQNYLNSIIDSMPSAL
IALDEQLYVTQWNQEASALSGTRLDEALNQPIFLAFEPLKPFLPQLRQTVEQHTVAKIER
VTWLKDEEPRHYALTFYPLMGGAGRGVVIRIDDITQRLSLEEMMVQSEKMLSVGGLAAGM
AHEINNPLGAILHNVQNIRRRLSPDLPKNLEQAEQLGIELETVNQYLKAREVPQLLDGIQ
QAGARAAKIVTHMLSFSRRSTRQMAPCDLPALIDQAVEIAGNDFDLAIGFDFKGQAIIRQ
FDPALGPVPGTANELEQVLLNLLKNAAQAIHQRTDDREPGRIILRTKLNPPWAEIQVEDN
GIGMSENVRKRTFEPFFTTKEIGQGTGLGLSVSYFIITNNHKGQMEVQSTPGQGTCFTLR
LPLSGTPVVSQELNQLSR