Protein Info for Pf1N1B4_639 in Pseudomonas fluorescens FW300-N1B4

Annotation: Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02075: RuvC
  • amino acids 4 to 150 (147 residues), 216 bits, E=1e-68
• TIGR00228: crossover junction endodeoxyribonuclease RuvC
  • amino acids 4 to 154 (151 residues), 202.5 bits, E=1.8e-64

Best Hits

Swiss-Prot: 96% identical to RUVC_PSEF5: Crossover junction endodeoxyribonuclease RuvC (ruvC) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01159, crossover junction endodeoxyribonuclease RuvC [EC: 3.1.22.4] (inferred from 94% identity to pst:PSPTO_3979)

MetaCyc: 58% identical to crossover junction endodeoxyribonuclease RuvC (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4)" in subsystem DNA-replication or RuvABC plus a hypothetical (EC 3.1.22.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10 or 3.1.22.4

Sequence Analysis Tools

See A0A161YZ03 at UniProt or InterPro

Protein Sequence (174 amino acids)

>Pf1N1B4_639 Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4) (Pseudomonas fluorescens FW300-N1B4)
MTLILGIDPGSRITGYGVVRDTGRGCVYVASGCIRTGSGELHERLQIVYRGVREVIQTYG
PVTMGIEKVFMARNADSALKLGQARGAAIVAGAEESLEIAEYTATQVKQAVTGTGGANKE
QVQMMVMHMLKLTSKPQIDASDALAIAICHAHTRSSLLPHGLGTARSRGGRLRL