Protein Info for Pf1N1B4_6035 in Pseudomonas fluorescens FW300-N1B4

Updated annotation (from data): D-ribose ABC transporter, substrate-binding component RbsB
Rationale: Specifically important for ribose utilization. Also involved in utilization of ribonucleotides, which might be hydrolyzed before uptake.
Original annotation: Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 318 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF00532: Peripla_BP_1" amino acids 35 to 297 (263 residues), 90.4 bits, see alignment E=2.2e-29 PF13407: Peripla_BP_4" amino acids 37 to 299 (263 residues), 197.1 bits, see alignment E=6.2e-62 PF13377: Peripla_BP_3" amino acids 171 to 262 (92 residues), 34.6 bits, see alignment E=3e-12

Best Hits

KEGG orthology group: K10439, ribose transport system substrate-binding protein (inferred from 94% identity to pfo:Pfl01_1919)

Predicted SEED Role

"Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1)" in subsystem D-ribose utilization (TC 3.A.1.2.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZH48 at UniProt or InterPro

Protein Sequence (318 amino acids)

>Pf1N1B4_6035 D-ribose ABC transporter, substrate-binding component RbsB (Pseudomonas fluorescens FW300-N1B4)
MKLPFAGRLLAVAMLAAASAALPVSSAFAETPEKPKVALVMKSLANEFFLTMEDGAKAYQ
KDHSGDFELISNGIKDETDTAGQTRIVEQMILSKVNALVIAPADSKAMVPVIKKAVDAGI
TVINIDNQLDPAVVKSKNITVPFVGPDNRKGARLVGEYLAKQLKAGDEVGIIEGVSTTTN
AQQRTAGFKDAMEAAQIKVVSLQSGDWEIDKGGKVASSMLSEYPNIKALLAGNDSMAVGA
VSAVRAAGKAGKVQVVGYDNINAIKPMLKDGRVLATADQFAARQAVFGIETALKIIKGET
VDSGANGVIETPVELVTK