Protein Info for Pf1N1B4_6023 in Pseudomonas fluorescens FW300-N1B4
Annotation: Threonyl-tRNA synthetase (EC 6.1.1.3)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 97% identical to SYT_PSEPF: Threonine--tRNA ligase (thrS) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 97% identity to pba:PSEBR_a3699)MetaCyc: 65% identical to threonine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Threonine--tRNA ligase. [EC: 6.1.1.3]; 6.1.1.3 [EC: 6.1.1.3]; 3.1.1.- [EC: 6.1.1.3]
Predicted SEED Role
"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)
MetaCyc Pathways
- tRNA charging (20/21 steps found)
- superpathway of chorismate metabolism (42/59 steps found)
- shinorine biosynthesis (3/8 steps found)
- vanchrobactin biosynthesis (2/8 steps found)
- enterobactin biosynthesis (4/11 steps found)
- acinetobactin biosynthesis (2/9 steps found)
- pseudomonine biosynthesis (2/9 steps found)
- bacillibactin biosynthesis (3/12 steps found)
- colibactin biosynthesis (6/38 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.1.1.3
Use Curated BLAST to search for 6.1.1.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166R3V3 at UniProt or InterPro
Protein Sequence (640 amino acids)
>Pf1N1B4_6023 Threonyl-tRNA synthetase (EC 6.1.1.3) (Pseudomonas fluorescens FW300-N1B4) MPTITLPDGSQRSFDHPVSVAEVAASIGAGLAKATVAGKVNGKLVDASDIIDSDSSLQII TPKDEEGLEIIRHSCAHLVGHAVKQLYPTAKMVIGPVIDEGFYYDIAFERPFTPDDLAAI EQRMQQLIEKDYDVIKKVTPRAEVIEVFKARGEDYKLRLVEDMPNEQAMGLYYHEEYVDM CRGPHVPNTRFLKSFKLTKLSGAYWRGDAKNEQLQRVYGTAWADKKQLAAYIQRIEEAEK RDHRKIGKRLGLFHTQEESPGMVFWHPNGWTLYQVLEQYMRKVQRDNGYLEIKTPQVVDR SLWEKSGHWANYADNMFTTQSENRDYAIKPMNCPCHVQVFNQGLKSYRELPMRLAEFGAC HRNEPSGALHGIMRVRAFTQDDAHIFCTEEQMQAESAAFIKLTMDVYADFGFKDVEMKLS TRPEKRVGSDELWDRAEAALAAALDSAGLPYDLQPGEGAFYGPKIEFSLKDCLGRVWQCG TLQLDFNLPVRLGAEYVSEDNSRKHPVMLHRAILGSFERFVGILIEHYEGAFPAWLAPTQ AVIMNITDKQADFAAEVEKTLNESGFRAKSDLRNEKIGFKIREHTLLKVPYLLVIGDREV EMQTVAVRTREGADLGSMPVAQFAEFLAQAVSRRGRPDSE