Protein Info for Pf1N1B4_600 in Pseudomonas fluorescens FW300-N1B4

Annotation: Glucokinase (EC 2.7.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02685: Glucokinase
  • amino acids 5 to 315 (311 residues), 358.7 bits, E=1.3e-111
• TIGR00749: glucokinase
  • amino acids 5 to 310 (306 residues), 257.1 bits, E=1.2e-80

Best Hits

Swiss-Prot: 54% identical to GLK_PSEA7: Glucokinase (glk) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K00845, glucokinase [EC: 2.7.1.2] (inferred from 94% identity to pfo:Pfl01_4374)

Predicted SEED Role

"Glucokinase (EC 2.7.1.2)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis (EC 2.7.1.2)

MetaCyc Pathways

• Bifidobacterium shunt (13/15 steps found)
• heterolactic fermentation (15/18 steps found)
• homolactic fermentation (10/12 steps found)
• sucrose degradation III (sucrose invertase) (4/4 steps found)
• glycolysis III (from glucose) (9/11 steps found)
• glycogen degradation II (5/6 steps found)
• trehalose degradation I (low osmolarity) (2/2 steps found)
• glucose and glucose-1-phosphate degradation (4/5 steps found)
• glycogen degradation I (6/8 steps found)
• sucrose biosynthesis II (6/8 steps found)
• GDP-α-D-glucose biosynthesis (2/3 steps found)
• trehalose degradation V (2/3 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• trehalose degradation II (cytosolic) (1/2 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• trehalose degradation IV (1/3 steps found)
• chitin biosynthesis (5/9 steps found)
• 1,3-propanediol biosynthesis (engineered) (4/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Starch and sucrose metabolism
• Streptomycin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.2

Sequence Analysis Tools

See A0A166MQQ4 at UniProt or InterPro

Protein Sequence (319 amino acids)

>Pf1N1B4_600 Glucokinase (EC 2.7.1.2) (Pseudomonas fluorescens FW300-N1B4)
LKLALVGDIGGTNARFALWKNQQLESVQVLATADHASPEEAIALYLSGLGLAPGSIGSVC
LSVAGPVSGDEFKFTNNHWRLSRQGFCKTLQVDQLLLVNDFSAMALGMTRLQPGEFRVVC
EGTPEPLRPAVVIGPGTGLGVGTLLDLGEGRFAPLPGEGGHVDLPLSSPRETQLWQHIFN
EIGHVSAETALSGSGLPRVYRAICAVDGHTPVLDTPEAITKAGLAGDPIALEVLEQFCCW
LGRVAGNNVLTTGARGGVYIVGGVIPRFADFFIESGFARCFADKGCMSDYFKGIPVWLVT
APYSGLVGAGVALEQSIPA