Protein Info for Pf1N1B4_5983 in Pseudomonas fluorescens FW300-N1B4

Annotation: Acyl-CoA dehydrogenase (EC 1.3.8.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 PF12418: AcylCoA_DH_N" amino acids 1 to 24 (24 residues), 23.7 bits, see alignment (E = 1e-08) PF02771: Acyl-CoA_dh_N" amino acids 32 to 148 (117 residues), 39.2 bits, see alignment E=2e-13 PF02770: Acyl-CoA_dh_M" amino acids 153 to 263 (111 residues), 48.2 bits, see alignment E=2.4e-16 PF00441: Acyl-CoA_dh_1" amino acids 273 to 444 (172 residues), 74.4 bits, see alignment E=2.8e-24 PF12806: Acyl-CoA_dh_C" amino acids 458 to 577 (120 residues), 122 bits, see alignment E=4.3e-39

Best Hits

KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 84% identity to pfo:Pfl01_5166)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.-

Use Curated BLAST to search for 1.3.8.7 or 1.3.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZH09 at UniProt or InterPro

Protein Sequence (582 amino acids)

>Pf1N1B4_5983 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Pseudomonas fluorescens FW300-N1B4)
MRFVLNEVFQVSRLWAQLPGLAEVIDEETAAAILEEAGKISGEVIAPLNRSSDEQGCTWS
NGAVQAPDGFVAAYQAFAEGGWVGVGGDPQFGGMGMPKAISAQVEEMVNSASLSFGLYPM
LTAGACLSINAHASQELKERYLPNMYAGTWTGSMCLTEAHAGTDLGLIRTRAEPQADGSF
KISGSKIFITGGEHDLTENIIHLVLARLPDAPAGPKGISLFLVPKVLVNDDGSLGERNSL
SCGSIEHKMGIKASATCVMNFDEATGWIVDAPNKGLAAMFTMMNYERLGVGIQGLALGER
SYQNAVEYARDRLQSRSPTGAQNKDKAADPIIVHPDVRRMLLTMKALNEGGRAFSSYVAL
QLDTAKYSDDAATRTRAQELVSLLTPVAKAFLTDMGLETTVHGQQIFGGHGYIREWGQEQ
LVRDVRITQIYEGTNGIQSLDLAGRKIVGSGGALYRLFASEIRNFTANAGAGLGEFCKPL
NAAVDTLDELTAWLLDRATHNPNEIGAASVEYLQVFGYTAYAYMWALMAKAAMGKQAEDD
FYASKLGTARFYFARLLPRIHSLSASVKAGSECLYELEPAHF