Protein Info for Pf1N1B4_5982 in Pseudomonas fluorescens FW300-N1B4

Annotation: Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 547 PF00501: AMP-binding" amino acids 31 to 405 (375 residues), 249.5 bits, see alignment E=5.2e-78 PF13193: AMP-binding_C" amino acids 455 to 535 (81 residues), 67.4 bits, see alignment E=1.7e-22

Best Hits

KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 74% identity to esc:Entcl_1369)

Predicted SEED Role

"Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-)" (EC 6.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.-

Use Curated BLAST to search for 6.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166R2Y7 at UniProt or InterPro

Protein Sequence (547 amino acids)

>Pf1N1B4_5982 Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-) (Pseudomonas fluorescens FW300-N1B4)
MMATKIIPPADGAYAYPLLIKQLLLSGVRYEPGREIVYADKLRYSYQTLNRRIRRLANAL
TAAGVKAGDTVALLDWDSHRYLECFFAVPMIGAVLHTVNIRLSPDQVLFTMNHAEDDLVL
VHDDFLPLVEQIHGRLETVKGYLQLTDDIATDTSLPVLGEYENLLSGSADQYDFPDFDEN
SVATLFYTTGTTGDPKGVYFTHRQLVLHTLNAVGTLGVYQGLPLLRSDDVYMPITPMFHV
HAWGVPYVATLMGLKQVYPGRYEPNSLVRLYREEKVTFSHCVPTILQMILSCEEAAQTRF
DGWKMLLGGSALTLGIASEASAKGIQVHSGYGMSETCPLLCLTYLRDEDLAQSTQTQLAT
RIKTGTPVPMVDLKIIDANGNDVAHDGESLGEIVVRAPWLTQGYLKEPEKGAELWHNGWM
HTGDLASIDALGGVEIKDRIKDVIKTGGEWISSLELESLISEHAAVMSVAVVGIADEQWG
ERPMALVVCEPGQYLDRKILEAHLQGFVERGRINKWAIPKQFKFVAEIPKTSVGKINKKL
IRESELS