Protein Info for Pf1N1B4_597 in Pseudomonas fluorescens FW300-N1B4

Updated annotation (from data): D-mannose isomerase (EC 5.3.1.7)
Rationale: Specifically important for: D-Mannose. Often annotated as N-acylglucosamine 2-epimerase, but this is also distantly related to the D-mannose isomerase Sama_0560. This organism has a fructokinase (Pf1N1B4_4844, no data) which probably utilizes the resulting fructose.
Original annotation: N-acylglucosamine 2-epimerase (EC 5.1.3.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07221: GlcNAc_2-epim
  • amino acids 40 to 394 (355 residues), 415.3 bits, E=1.1e-128

Best Hits

Swiss-Prot: 49% identical to SQUS_ECOLI: Sulfoquinovose isomerase (yihS) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 89% identity to pfo:Pfl01_4371)

MetaCyc: 49% identical to sulfoquinovose isomerase (Escherichia coli K-12 substr. MG1655)
Mannose isomerase. [EC: 5.3.1.7]; RXN-15296 [EC: 5.3.1.7, 5.3.1.31]

Predicted SEED Role

"N-acylglucosamine 2-epimerase (EC 5.1.3.8)" in subsystem Sialic Acid Metabolism (EC 5.1.3.8)

MetaCyc Pathways

• N-acetylneuraminate and N-acetylmannosamine degradation II (1/3 steps found)
• sulfoquinovose degradation III (1/4 steps found)
• sulfoquinovose degradation I (1/5 steps found)
• sulfoquinovose degradation VI (1/5 steps found)
• sulfoquinovose degradation V (1/7 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.8 or 5.3.1.31 or 5.3.1.7

Sequence Analysis Tools

See A0A161YYW9 at UniProt or InterPro

Protein Sequence (419 amino acids)

>Pf1N1B4_597 D-mannose isomerase (EC 5.3.1.7) (Pseudomonas fluorescens FW300-N1B4)
MDTFQPAFSSWLNAPAHQQWLAAEGLRLLAFAKAAKLAEGFGNLDEKGRLAANAQAETMN
TARMTHSFAMAHIQGLPGFAELVDHGIQALSGPLRDAEHGGWFATPEHRDGNTGKAAYLH
AFVALAASSAVVAQRPGAQALLDDAIHIIDSHFWSEEEGAMRESFNRDWSVEEAYRGANS
NMHATEAFLALADVTEDNRWLIRAQRIVERVIHDHAAVNDYLVVEHFDRDWQPLRDYNYD
NPADGFRPYGTTPGHGFEWARLLLHLEAARVQAGILTPGWLATDAQKLFDHNCRHGWDVD
GAPGIVYTLDWDNRAVVRHRLHWTHAEASAAASALLKRTGDEQYERWYRLFWEFCDSHFI
DRCDGSWHHELDPLNRPSADIWAGKPDLYHAWQAVLIPRLPLAPSMAIALAQLSQSVAV