Protein Info for Pf1N1B4_5956 in Pseudomonas fluorescens FW300-N1B4

Annotation: NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 54% identical to G3P1_BACCE: Glyceraldehyde-3-phosphate dehydrogenase 1 (gap1) from Bacillus cereus

KEGG orthology group: K00134, glyceraldehyde 3-phosphate dehydrogenase [EC: 1.2.1.12] (inferred from 94% identity to pba:PSEBR_a3680)

MetaCyc: 91% identical to glyceraldehyde-3-phosphate dehydrogenase (arsenate-transferring) (Pseudomonas aeruginosa)
RXN1YI0-11 [EC: 1.2.1.107]

Predicted SEED Role

"NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)" in subsystem Calvin-Benson cycle or Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Pyridoxin (Vitamin B6) Biosynthesis or Redox-dependent regulation of nucleus processes (EC 1.2.1.12)

MetaCyc Pathways

superpathway of glucose and xylose degradation (16/17 steps found)
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
Bifidobacterium shunt (13/15 steps found)
heterolactic fermentation (15/18 steps found)
pyridoxal 5'-phosphate biosynthesis I (7/7 steps found)
superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
gluconeogenesis I (11/13 steps found)
Entner-Doudoroff pathway I (8/9 steps found)
formaldehyde assimilation III (dihydroxyacetone cycle) (10/12 steps found)
homolactic fermentation (10/12 steps found)
superpathway of anaerobic sucrose degradation (15/19 steps found)
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
glycolysis II (from fructose 6-phosphate) (9/11 steps found)
glycolysis III (from glucose) (9/11 steps found)
glycolysis IV (8/10 steps found)
glycolysis I (from glucose 6-phosphate) (10/13 steps found)
arsenate detoxification V (2/2 steps found)
sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
gluconeogenesis III (8/12 steps found)
glycolysis VI (from fructose) (7/11 steps found)
glycerol degradation to butanol (10/16 steps found)
superpathway of hexitol degradation (bacteria) (11/18 steps found)
superpathway of N-acetylneuraminate degradation (13/22 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.12

Use Curated BLAST to search for 1.2.1.107 or 1.2.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166R297 at UniProt or InterPro

Protein Sequence (350 amino acids)

>Pf1N1B4_5956 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) (Pseudomonas fluorescens FW300-N1B4)
MIPKAPPLTTETEHTNMTIKVGINGFGRIGRLALRAAWRWPEFEFVQINDPAGDAATHAH
LLNFDSVHGRWNDQADSEGDSIVIDGKRIKVTANKAIADTDWSGCDLVIEASGKMKTVAV
LQAYLDQGVKRVVVSAPVKEQGALNIVMGVNHQLFNPAEHRIVTAASCTTNCLAPVVKVI
HEKLGIRHGSITTIHDLTNTQSILDQPHKDLRRARASGMSLIPTSTGSATAIAEIFPELR
GRLNGHAVRVPLANASLTDCVFEVERVTTVEEVNQFLKDASENELKDILGFEERPLVSID
YRTDPRSSIIDALSTMVINGTQVKLYAWYDNEWGYANRTVELARMVGLAV