Protein Info for Pf1N1B4_5870 in Pseudomonas fluorescens FW300-N1B4

Annotation: VgrG protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 680 TIGR03361: type VI secretion system Vgr family protein" amino acids 10 to 524 (515 residues), 685.8 bits, see alignment E=3.6e-210 TIGR01646: Rhs element Vgr protein" amino acids 22 to 506 (485 residues), 434.2 bits, see alignment E=7.1e-134 PF05954: Phage_GPD" amino acids 29 to 322 (294 residues), 275.1 bits, see alignment E=7.2e-86 PF04717: Phage_base_V" amino acids 388 to 455 (68 residues), 52.7 bits, see alignment E=4.5e-18

Best Hits

KEGG orthology group: K11904, type VI secretion system secreted protein VgrG (inferred from 92% identity to pst:PSPTO_5436)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166R0H3 at UniProt or InterPro

Protein Sequence (680 amino acids)

>Pf1N1B4_5870 VgrG protein (Pseudomonas fluorescens FW300-N1B4)
MFAPANQTHFALTVEGLENDLQVLSLQGREAISQPFVFEVELVSEQPSLDLETLLHKPAF
LQLSPDGSGIHGQIYRAAQGDSGKRLTRYAVTLRPQLSYLAHRINQRIFQNLSVPKIIGI
VLEEHGIQSNAYEFKVGAIYPERIYCVQYDESDLQFIQRLCEEEGIHYHFQHSATAHKLV
FGDDQTVFPKLAPVAYQQDSGMVANDPVIKRFDLRLETRTSRTTRRDYDFEKPRLTLESE
HRGDALPDLEDYDYPGRFIDRERGKHLAKRALERHRSDFQLVEGKSDQPLLVSGHFLALT
QHPKAKWNDLWLLTEILHEGKQPQVLEESVTSSTTNLKDDFHQGYRNRFQATPWDVPNRP
PLNHPKPRILGSQSAVVTGPKGEEIHCDQYGRVKVQFHWDREGQADDKTSCWLRVSSAWA
GAHYGGIAIPRIGMEVLVSFLEGDPDQPLISGCLYHKENVVPYALPANKTRSTFKTLSSP
GGGGFNELRIEDKKGQEQIFLHAQRDWDENVEHDQKIRVGNERHDTVEQNSYSEFKAEEH
HTVYADRKVEARANDHLTVGVNQHIKVGTGQFIEAGQEIHLSSGMKVVLEAGSELTLKGG
GSFIKIDASGVTLSGPVINMNSGGSPGNGTGAAPLMPGALKQADADKAGAVLSPAQINTL
KRNAPFCEECEKCKAGACAI