Protein Info for Pf1N1B4_5860 in Pseudomonas fluorescens FW300-N1B4

Annotation: Two-component response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 transmembrane" amino acids 30 to 50 (21 residues), see Phobius details amino acids 70 to 94 (25 residues), see Phobius details amino acids 100 to 121 (22 residues), see Phobius details PF05230: MASE2" amino acids 1 to 55 (55 residues), 72.7 bits, see alignment E=2.1e-24 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 137 to 297 (161 residues), 171.1 bits, see alignment E=8.5e-55 PF00990: GGDEF" amino acids 140 to 294 (155 residues), 157.1 bits, see alignment E=3.3e-50

Best Hits

KEGG orthology group: None (inferred from 82% identity to pfo:Pfl01_2049)

Predicted SEED Role

"Two-component response regulator"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161XNN7 at UniProt or InterPro

Protein Sequence (311 amino acids)

>Pf1N1B4_5860 Two-component response regulator (Pseudomonas fluorescens FW300-N1B4)
MLFNGLLWPHVAYQWARRAEVPFRAEHRNILVDAFLGGFWVAAMQFNPLPSAATLSMMAM
HNVAIGGVRFLLVGAVAQVLGIGLGLLVFMPAFVPLTSPFQLYACLPLLSIYPLSLGWIC
FRQADTLGRHKRELLALSRTDSLTGLLNHGAWKDQLEIEFQRCQRQQQGGAIALIDIDHF
KTINDTYGHVAGDIVLRQLSKMLKQNLRAADVAGRYGGDEFCVLLPDLPLDRAAVAMDAL
RDRFATLGYEQNPALKVSLSIGLAAFNPAHDDATLWLNDADQALYEAKSTGRNRVICHSD
GKPHKALFDSV