Protein Info for Pf1N1B4_5774 in Pseudomonas fluorescens FW300-N1B4

Annotation: Nitrite reductase associated c-type cytochorome NirN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 497 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF13442: Cytochrome_CBB3" amino acids 25 to 96 (72 residues), 44.1 bits, see alignment E=6.5e-15 PF00034: Cytochrom_C" amino acids 26 to 98 (73 residues), 24.4 bits, see alignment E=1.9e-08 PF02239: Cytochrom_D1" amino acids 133 to 488 (356 residues), 481.5 bits, see alignment E=3.7e-148

Best Hits

KEGG orthology group: None (inferred from 84% identity to pba:PSEBR_a3181)

Predicted SEED Role

"Nitrite reductase associated c-type cytochorome NirN" in subsystem Dissimilatory nitrite reductase

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QY80 at UniProt or InterPro

Protein Sequence (497 amino acids)

>Pf1N1B4_5774 Nitrite reductase associated c-type cytochorome NirN (Pseudomonas fluorescens FW300-N1B4)
MRRMLLAPLLWIGAVHAGAALPSSDAAADLYQQHCQRCHGANRFGGTGPALLPESLSRIK
PDEIRSVIQNGRPASQMTGYANVFNPAQIDALVSFLQQPPATPPTWNNDDIRGSHSILAD
ISKLPDTPQHGADPLNLFVVVEAGDHHIDILDGDRFEVLARFASHFAVHGGPKFSPDGRF
VYVASRDGWVSLYDLHNLKLIAEVRAGLNTRNLAVSKDGRWVLVGNYLPGNLVILDARDL
SLVKTIPAIGQDGTASRVSAVYTAPPRDSFIVALKDVKEVWELSSAGTPDFEPRRIKAED
VLDDFSFSPDYKQLLATSRKAKGGQVIDLDSGKVVTDIPLPGMPHLGSGTYWKRNGEWVF
ATPNISKGLISVIDFKTWKLIKEIPTLGPGFFMRSHVNSRYAWTDVFFGPDNDAIHLIDK
QTLEVAHTLRPMPGKTAAHVEFTRDGRYLLLSIWATDGALIVYDSNTLKEIKRLPMNKPS
GKYNVGNKIEFVEGTSH