Protein Info for Pf1N1B4_5770 in Pseudomonas fluorescens FW300-N1B4

Annotation: Heme d1 biosynthesis protein NirG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 74% identical to NIRG_PSEST: Protein NirG (nirG) from Pseudomonas stutzeri

KEGG orthology group: None (inferred from 84% identity to pba:PSEBR_a3177)

MetaCyc: 46% identical to siroheme decarboxylase NirG subunit (Paracoccus pantotrophus)
RXN-15805 [EC: 4.1.1.111]

Predicted SEED Role

"Heme d1 biosynthesis protein NirG" in subsystem Dissimilatory nitrite reductase or Heme biosynthesis orphans

MetaCyc Pathways

heme d₁ biosynthesis (2/3 steps found)
heme b biosynthesis III (from siroheme) (1/3 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.111

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZGK9 at UniProt or InterPro

Protein Sequence (147 amino acids)

>Pf1N1B4_5770 Heme d1 biosynthesis protein NirG (Pseudomonas fluorescens FW300-N1B4)
MDDLDRRLINRLQLGLPLVRHPWQALAAELESSSTELLDRLHELLEDGMLTRFGPMFDIE
RLGGAFTLAALAVPEERFEDVAEQLQALPEVAHNYRREHAWNMWFVLGCPTEQGIAETLQ
HIENLTGLPLLNLPKEETYHVGLYFPV