Protein Info for Pf1N1B4_577 in Pseudomonas fluorescens FW300-N1B4

Annotation: Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 314 PF12146: Hydrolase_4" amino acids 27 to 288 (262 residues), 187.8 bits, see alignment E=4e-59 PF00561: Abhydrolase_1" amino acids 30 to 285 (256 residues), 59.7 bits, see alignment E=7.3e-20 PF12697: Abhydrolase_6" amino acids 31 to 287 (257 residues), 72.3 bits, see alignment E=1.9e-23 PF00326: Peptidase_S9" amino acids 224 to 305 (82 residues), 22.7 bits, see alignment E=1.2e-08

Best Hits

KEGG orthology group: None (inferred from 88% identity to pfo:Pfl01_4355)

Predicted SEED Role

"Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative" (EC 3.1.1.23, EC 3.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5

Use Curated BLAST to search for 3.1.1.23 or 3.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MQ88 at UniProt or InterPro

Protein Sequence (314 amino acids)

>Pf1N1B4_577 Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative (Pseudomonas fluorescens FW300-N1B4)
MIHDTFWLTASDRSRLFVNQWLPAAPLKAVILLAHGMAEHSGRYARLAQACCDRDYGVYA
PDLRGHGKTAENGTLGHFADDDGWCKVVGDLASLNQHIGQQHPGVPIVLLGHSMGSYIAQ
AYLLHHSASLHGAILSGSNFQPVTLYRAARQIARLERLRQGPKGLSALIEWLSFGSFNKK
FKPARTPFDWLSRDPTEVDKYANDPLCGFRCTNQLWIDLLGGLQQISKASNLAQIDPGLP
LLVIGGECDPVSEGKRLKDLAHALRDAGCQNLQLTIYPQARHELFNEINRDEVTADVLNW
IAQDLSARRPPRTE