Protein Info for Pf1N1B4_5729 in Pseudomonas fluorescens FW300-N1B4

Annotation: Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 671 PF03477: ATP-cone" amino acids 15 to 97 (83 residues), 59.6 bits, see alignment E=3.5e-20 TIGR02487: anaerobic ribonucleoside-triphosphate reductase" amino acids 126 to 656 (531 residues), 453.9 bits, see alignment E=4.2e-140 PF13597: NRDD" amino acids 127 to 657 (531 residues), 565 bits, see alignment E=2.1e-173

Best Hits

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 92% identity to pba:PSEBR_a3142)

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161XND9 at UniProt or InterPro

Protein Sequence (671 amino acids)

>Pf1N1B4_5729 Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2) (Pseudomonas fluorescens FW300-N1B4)
MQSTLISVGCNRLHKRDGSLVAFDADKIRQALIAAGKATGEYTGVEVEGLLQAVLARLDG
LPRLHVEQIQDRVERVLMDAGFFFAMRAYIVYREQHGRLRRDRRTMVEVATSMNEYLDRE
DWRVQANANQGYSLGGLVLNVSGKVTANYWLDEVYSEAIGQAHREADLHVHDLDMLAGYC
AGWSLRTLLHEGLNGVPGRVEAGPPKHLSSALGQMVNFLGTLQNEWAGAQAFSSFDTYLA
PYVRKDQLSYQEVRQAIQEFIYNLNVPSRWGTQTPFTNLTFDWVCPQDLREQIPVIGGKE
MPFAYGDLQVEMDLLNRAYIEVMQAGDAKGRVFTFPIPTYNITHDFPWDSENADRLFEMT
ARYGLPYFQNFLNSDLQPNQVRSMCCRLQLDVRELLKRGGGLFGSAEQTGSLGVVTINCA
RLGYVFKGNTSGLLQRLDALMELAMESLEVKRKVIQHHMDAGLYPYTKRYLGTLRNHFST
IGLNGLHEMLRNFTGDEEGMHTEHGRQFALKMLDHVRATLLRFQEETGHLYNLEATPAEG
TTYRFAKEDLKRYPDILQAGSVQAPYYTNSSQLPVGYTEDPFEALELQDELQCKYTGGTV
LHLYMAERISSTQACKQLVRKALGRFRLPYLTVTPTFSICPVHGYLDGEHEFCPKCDEVL
LLQEQKAGAVH