Protein Info for Pf1N1B4_572 in Pseudomonas fluorescens FW300-N1B4

Annotation: Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 341 transmembrane" amino acids 36 to 57 (22 residues), see Phobius details amino acids 76 to 94 (19 residues), see Phobius details PF00501: AMP-binding" amino acids 1 to 194 (194 residues), 163 bits, see alignment E=9.6e-52 PF13193: AMP-binding_C" amino acids 245 to 320 (76 residues), 69.7 bits, see alignment E=3.4e-23

Best Hits

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (341 amino acids)

>Pf1N1B4_572 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) (Pseudomonas fluorescens FW300-N1B4)
MLTHRNLVANMLQCKALMGSNLNEGCEILITPLPLYHIYAFTFHCMAMMLIGNHNILISN
PRDLTAMVKELSKWKFSGFVGLNTLFVALCNNEAFRKLDFSSLKITLSGGMALQLAAAER
WKEVAGCPICEGYGMTETSPVATVNPSQNIQIGTIGIPVPSTLCKVIDDAGVEQPLGAIG
ELCVKGPQVMKGYWQRQDATDEILDSEGWLKTGDIALIQPDGYMRIVDRKKDMILVSGFN
VYPNELEDVLATLPGVLQCAAIGVPDEKSGEAIKIFIVAKPGVTLTKDQVMEHMRANVTG
YKVPKAVEFRDALPTTNVGKILRRELRDEELKKLGVKKVSA