Protein Info for Pf1N1B4_57 in Pseudomonas fluorescens FW300-N1B4

Annotation: tRNA dihydrouridine synthase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 83% identical to DUSA_PSESM: tRNA-dihydrouridine(20/20a) synthase (dusA) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K05539, tRNA-dihydrouridine synthase A [EC: 1.-.-.-] (inferred from 95% identity to pfo:Pfl01_3824)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MED7 at UniProt or InterPro

Protein Sequence (312 amino acids)

>Pf1N1B4_57 tRNA dihydrouridine synthase A (Pseudomonas fluorescens FW300-N1B4)
MMDWTDRHCRFFLRLLSKHALLYTEMVTTGALLNGDHDRFLRHNEAEHPLALQLGGSVPL
DLAACARMAQDHGYDEVNLNVGCPSDRVQNNMIGACLMGHPQLVADCVKAMRDAVSIPVT
VKHRIGINGRDSYEQLCDFVGTVRDAGCTSFTVHARIAILEGLSPKENRDIPPLRYDVAA
RLKTDFPELEIILNGGIKTLEACHEHLQTFDGVMLGREAYHNPYVMAEVDQQLFGSTAPV
ISRAEALAQLRPYIAEHLLAGGSMHHITRHVLGLGTGFPGARKFRQLLSVDIHKAKEPLA
LLDQAAELLEGR