Protein Info for Pf1N1B4_5684 in Pseudomonas fluorescens FW300-N1B4
Annotation: Glyceraldehyde-3-phosphate dehydrogenase, putative
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00131, glyceraldehyde-3-phosphate dehydrogenase (NADP) [EC: 1.2.1.9] (inferred from 92% identity to pfo:Pfl01_2492)Predicted SEED Role
"Glyceraldehyde-3-phosphate dehydrogenase, putative"
MetaCyc Pathways
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
- glycolysis IV (8/10 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.2.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166QWI2 at UniProt or InterPro
Protein Sequence (541 amino acids)
>Pf1N1B4_5684 Glyceraldehyde-3-phosphate dehydrogenase, putative (Pseudomonas fluorescens FW300-N1B4) MTTAQILGNLFPTADNVPEKYRLNGPTEQREYLVDGELKTWSGPLAQVRSPVYLSGANGD EQVILGSTPLLDADTALTALDAAVRAYDRGQGLWPTMRVAERIQHVETFLARMREQREAV VKLLMWEIGKNLKDSEKEFDRTCDYIVDTVNALKELDRRSSRFELEQDTLGQIRRVPLGV ALCMGPYNYPLNETFTTLIPALIMGNTVVFKPAKLGVLLIRPLLEAFRDSFPAGVINVIY GSGRETVSALMASGKIDIFAFIGTNKAASDLKKLHPRPHRLRAALGLDAKNPGIVLPEVD LDNAVSEAVTGSLSFNGQRCTALKILFVHEDVVEAFIEKFNAKLADLKPGMPWDSGVALT PLPESGKVDYLHTLVADAVSKGAAVVNPNGGEARASFFYPAVLYPVTPQMRVYQEEQFGP VVPIVPYRHLDTVIDYVLESDFGQQLSIFGTNPVAVGRLVDTFANQVGRINLNAQCQRGP DTYPFNGRKNSAEGTLSVHDALRVFSIRTLVATKFQESSKELLSEIISGRNSSFLTTDYI F