Protein Info for Pf1N1B4_5639 in Pseudomonas fluorescens FW300-N1B4
Annotation: D-serine deaminase (EC 4.3.1.18)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 92% identity to pfo:Pfl01_3086)MetaCyc: 87% identical to glucosaminate ammonia-lyase (Pseudomonas simiae)
Glucosaminate ammonia-lyase. [EC: 4.3.1.9]
Predicted SEED Role
"D-serine deaminase (EC 4.3.1.18)" (EC 4.3.1.18)
MetaCyc Pathways
- glycine betaine degradation III (7/7 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
- D-serine degradation (3/3 steps found)
- L-cysteine degradation II (3/3 steps found)
- L-serine degradation (3/3 steps found)
- glycine betaine degradation I (6/8 steps found)
- L-tryptophan degradation II (via pyruvate) (2/3 steps found)
- L-methionine biosynthesis II (4/6 steps found)
- purine nucleobases degradation II (anaerobic) (16/24 steps found)
- felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
- L-mimosine degradation (4/8 steps found)
- glutathione-mediated detoxification I (3/8 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.3.1.18 or 4.3.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A166QVF3 at UniProt or InterPro
Protein Sequence (405 amino acids)
>Pf1N1B4_5639 D-serine deaminase (EC 4.3.1.18) (Pseudomonas fluorescens FW300-N1B4) MYSAKNTAAVEKGFAHTGANLVRDVSLPALVLHRDALEHNIRWMQDFVSNSGAELAPHGK TSMTPALFRRQLDAGAWGITLASATQTRAAYAHGVHRVLMANQLVGTPNMALIADLLADP TFDFYCMVDHPDNVADLGAYFASRGVRLNVMIEYGVVGGRCGCRTETEVLALAKAIAAQP ALALTGIEGYEGVIHGDHAVSGIRAFADSLVRLAVQLQDSGAFAIAKPIITASGSAWYDL IAESFEAQNACGRFLSVLRPGSYVAHDHGIYKEAQCCVLDRRSDLHEGLRPALEVWAHVQ SLPEPGFAVIALGKRDVAYDAGLPVPLLRYKAGVVPAIGEDVGACKVTAVMDQHAFMTVA PGVELRVGDIISFGTSHPCLTFDKWRIGCLVDEQLNVIETMETCF