Protein Info for Pf1N1B4_563 in Pseudomonas fluorescens FW300-N1B4

Annotation: RNA polymerase sigma-70 factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 182 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 15 to 160 (146 residues), 64.6 bits, see alignment E=4.2e-22 PF04542: Sigma70_r2" amino acids 15 to 80 (66 residues), 40.4 bits, see alignment E=3e-14 PF08281: Sigma70_r4_2" amino acids 112 to 159 (48 residues), 54.2 bits, see alignment E=1.3e-18 PF13384: HTH_23" amino acids 123 to 154 (32 residues), 25 bits, see alignment 2e-09

Best Hits

Swiss-Prot: 42% identical to FECI_ECOLI: Probable RNA polymerase sigma factor FecI (fecI) from Escherichia coli (strain K12)

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 73% identity to pfo:Pfl01_4345)

MetaCyc: 42% identical to RNA polymerase sigma factor FecI (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"RNA polymerase sigma-70 factor" in subsystem Transcription initiation, bacterial sigma factors

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MQ44 at UniProt or InterPro

Protein Sequence (182 amino acids)

>Pf1N1B4_563 RNA polymerase sigma-70 factor (Pseudomonas fluorescens FW300-N1B4)
MSGADLSHRNDVGGLFRAHYAWLCTRLRRYLGASPSVEDIAAETFVQLLESPGLTPIREP
RALLTTIAHRLIYQLWRRRDLERQHLDQLSPIDPLGGPSPEDLAQFSQTLTRLDRTLERL
PGKVRAAFLLSRIDGLTYPQIAAELGISQRSVSVYMTRSQALCAQHSANEPLNRPPLTQR
SA