Protein Info for Pf1N1B4_5606 in Pseudomonas fluorescens FW300-N1B4

Annotation: Alpha-ketoglutarate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 transmembrane" amino acids 38 to 59 (22 residues), see Phobius details amino acids 68 to 92 (25 residues), see Phobius details amino acids 100 to 121 (22 residues), see Phobius details amino acids 125 to 148 (24 residues), see Phobius details amino acids 169 to 189 (21 residues), see Phobius details amino acids 201 to 220 (20 residues), see Phobius details amino acids 250 to 268 (19 residues), see Phobius details amino acids 287 to 309 (23 residues), see Phobius details amino acids 317 to 336 (20 residues), see Phobius details amino acids 343 to 365 (23 residues), see Phobius details amino acids 376 to 397 (22 residues), see Phobius details amino acids 411 to 429 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 33 to 227 (195 residues), 93.9 bits, see alignment E=1.1e-30 amino acids 228 to 427 (200 residues), 26.3 bits, see alignment E=3.5e-10 PF07690: MFS_1" amino acids 46 to 283 (238 residues), 52.5 bits, see alignment E=3.8e-18 amino acids 280 to 430 (151 residues), 37 bits, see alignment E=2e-13

Best Hits

Swiss-Prot: 58% identical to KGTP_SHIFL: Alpha-ketoglutarate permease (kgtP) from Shigella flexneri

KEGG orthology group: None (inferred from 94% identity to psb:Psyr_2179)

MetaCyc: 58% identical to alpha-ketoglutarate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-23

Predicted SEED Role

"Alpha-ketoglutarate permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166R4M4 at UniProt or InterPro

Protein Sequence (461 amino acids)

>Pf1N1B4_5606 Alpha-ketoglutarate permease (Pseudomonas fluorescens FW300-N1B4)
VNNMSASITDAKDHAAETAPMKAPNRVKAIMGACSGNLVEWYDFFIYAYTAIYFAASFFP
KGDTTSQLLATAGVFAVGFFMRPLGGWIFGWIADTRGRKVSMIISVFMMCAGSLLIAVMP
TYATIGVAAPILLVVARLIQGLSVGAEYGTGATYISEIATPGRRCFYGSFQYFTIIAGQL
LALMTVVILQQTLTGEELREWGWRIPFFIGALSSIVVVYLRRAMHETATKKDMNRKDAGS
LRGMFKHKRAVALVVAFTIGGSLYFYTFTTYMQKFLVISAGFSPETVSFIMTAALVGFMF
CQPLFGLLADRIGIKAHMLMFSGLAMLLVIPLLYSLQSVSSPFTAFLLVFGGLAIASLYT
PIAGIVKAELFPPAVRALGVGFPYAVGNAAFGGTAEYVALSLRSQGVEAYFFFYVAAVVT
ITFIAAMLMPNLSRYGYISGSGEIEERTGLKGCSKPSAARV