Protein Info for Pf1N1B4_5603 in Pseudomonas fluorescens FW300-N1B4

Annotation: Threonine dehydrogenase and related Zn-dependent dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 TIGR02819: formaldehyde dehydrogenase, glutathione-independent" amino acids 2 to 395 (394 residues), 754.7 bits, see alignment E=8.3e-232 PF08240: ADH_N" amino acids 35 to 145 (111 residues), 94.3 bits, see alignment E=2e-31

Best Hits

Swiss-Prot: 91% identical to FADH_PSEPU: Glutathione-independent formaldehyde dehydrogenase (fdhA) from Pseudomonas putida

KEGG orthology group: K00148, glutathione-independent formaldehyde dehydrogenase [EC: 1.2.1.46] (inferred from 90% identity to pfo:Pfl01_5202)

Predicted SEED Role

"Threonine dehydrogenase and related Zn-dependent dehydrogenases" in subsystem Threonine degradation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.46

Use Curated BLAST to search for 1.2.1.46

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161ZG90 at UniProt or InterPro

Protein Sequence (399 amino acids)

>Pf1N1B4_5603 Threonine dehydrogenase and related Zn-dependent dehydrogenases (Pseudomonas fluorescens FW300-N1B4)
MSGNRGVVYLGAGKVEVQTIPYPKMEDPRGKRIDHGVILRVVSTNICGSDQHMVRGRTTA
QVGLVLGHEITGEVIEKGRDVENVQIGDLVSVPFNVACGRCRSCKEQHTGVCLTVNPARP
GGAYGYVDMGDWVGGQAEYVLVPYADFNLLKLPDRDKAMEKIRDLTCLSDILPTGYHGAV
TAGVGPGSTVYIAGAGPVGLAAAASARLLGAAVVIVGDVNPLRLAHAKAQGFEIADLTLD
TPLHEQIANMLGEPEVDCAVDAVGFEARGHGHDGAQHEAPADVLNALMGVVRVAGNIGIP
GLYVTEDPGAVNKAAKHGSLNIRFGLGWAKSHSFHTGQTPTMKYNRQLMQAIMWDRIKIA
EVVGVQVISLDDAPRGYHEFDAGAPKKFVMDPHKLFTAA