Protein Info for Pf1N1B4_516 in Pseudomonas fluorescens FW300-N1B4

Annotation: Sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 70 to 92 (23 residues), see Phobius details PF00672: HAMP" amino acids 92 to 141 (50 residues), 40.3 bits, see alignment 4.7e-14 PF00512: HisKA" amino acids 146 to 196 (51 residues), 30.5 bits, see alignment 4.7e-11 PF02518: HATPase_c" amino acids 244 to 349 (106 residues), 87.2 bits, see alignment E=1.5e-28

Best Hits

KEGG orthology group: None (inferred from 86% identity to pfo:Pfl01_4198)

Predicted SEED Role

"Sensor histidine kinase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166MP29 at UniProt or InterPro

Protein Sequence (350 amino acids)

>Pf1N1B4_516 Sensor histidine kinase (Pseudomonas fluorescens FW300-N1B4)
MRTRFDTLFGRLFGVLLLAIVLAHLLAFFWFRHYGPPPPPPAPSEFSERFDGQRPPPDPR
FGNRPPRPWFGGPLVPLTFQFVSLIIAAWYGAKLLTRPIQRLSDAAERLSENLDSPPLDE
SGPREARQAAHTFNLMQKRILEQVQQRSRMLGAVSHDLRTPLSRLKLRLEQIDDDKLQGQ
MRQDLNDMISMLDSTLTYLHEQRTSEAPQWMDVQALVESLSENAQDQGSNVQASGHCAPL
QVQPMALRSCINNLLDNALRYAGDALITLEDSREELVIRVIDHGPGIAADKREAVFEPFF
RLEGSRNRNSGGVGLGMTIAREAAERLGGQLSLEETSGGGLTAVIRLPRL