Protein Info for Pf1N1B4_5139 in Pseudomonas fluorescens FW300-N1B4

Annotation: Gamma-aminobutyrate permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 501 transmembrane" amino acids 55 to 77 (23 residues), see Phobius details amino acids 82 to 101 (20 residues), see Phobius details amino acids 125 to 148 (24 residues), see Phobius details amino acids 162 to 181 (20 residues), see Phobius details amino acids 191 to 214 (24 residues), see Phobius details amino acids 235 to 258 (24 residues), see Phobius details amino acids 278 to 299 (22 residues), see Phobius details amino acids 324 to 348 (25 residues), see Phobius details amino acids 370 to 391 (22 residues), see Phobius details amino acids 397 to 417 (21 residues), see Phobius details amino acids 438 to 460 (23 residues), see Phobius details amino acids 467 to 486 (20 residues), see Phobius details TIGR01773: GABA permease" amino acids 42 to 488 (447 residues), 688.7 bits, see alignment E=1.6e-211 PF00324: AA_permease" amino acids 54 to 465 (412 residues), 397.7 bits, see alignment E=7e-123 PF13520: AA_permease_2" amino acids 55 to 472 (418 residues), 129.8 bits, see alignment E=1.4e-41

Best Hits

Swiss-Prot: 70% identical to BAUD_PSEAE: Probable GABA permease (bauD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03293, amino acid transporter, AAT family (inferred from 93% identity to pfo:Pfl01_2293)

MetaCyc: 60% identical to 4-aminobutanoate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-384; TRANS-RXN-57

Predicted SEED Role

"Gamma-aminobutyrate permease"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QG80 at UniProt or InterPro

Protein Sequence (501 amino acids)

>Pf1N1B4_5139 Gamma-aminobutyrate permease (Pseudomonas fluorescens FW300-N1B4)
VRRVRDAATGKNDDRLTLSQYALTSITREQKKTMNNLNSRDSNGQLAQGFKPRHVTMLSI
AGIIGAGLFVGSGHAIAAAGPAVLLAYLFSGLLVVLVMRMLGEMAVANPDTGSFSTYADQ
AIGRWAGFTIGWLYWWFWVLVIPIEALAAGHVLNQWFPQIDTWLFALTSIILLVVTNLFS
VSKYGEFEFWFAMAKVVAIIGFIGLGFAVLMGWIPEREASGLSRLMEEHGGFAPNGLSAV
VGAFITIMFSFIGTEAVTIAAAESSNPAQNIAKATRSVIWRIGVFYLLSIFVVISVVPWN
DPLLASVGSYQRALELMNIPHAKLMVDVVVLIAVASCMNSSIYIASRMLFSLGKRGDAPK
PLKVTSSDGVPRAAVIASTVLGAGVTLFSYFMPAGLFQFLLASSGAIALLVYLVIAVSQL
RMRKILLRQNVTLTFKMWLFPWLTWLVIAFICAALAVMMVTPEHRMEVSSTIGLALVISF
IGLVTARQHGQPRSAVSAESA