Protein Info for Pf1N1B4_5116 in Pseudomonas fluorescens FW300-N1B4

Updated annotation (from data): Sucrose alpha-glucosidase (EC 3.2.1.48)
Rationale: Specifically important for utilizing Sucrose. Automated validation from mutant phenotype: the predicted function (3.2.1.48-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 489 TIGR01322: sucrose-6-phosphate hydrolase" amino acids 19 to 458 (440 residues), 445.9 bits, see alignment E=7.3e-138 PF00251: Glyco_hydro_32N" amino acids 28 to 329 (302 residues), 349.3 bits, see alignment E=2.5e-108 PF08244: Glyco_hydro_32C" amino acids 358 to 478 (121 residues), 104.8 bits, see alignment E=4.7e-34

Best Hits

KEGG orthology group: K01193, beta-fructofuranosidase [EC: 3.2.1.26] (inferred from 87% identity to pba:PSEBR_a2364)

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.26 or 3.2.1.48 or 3.2.1.B3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QFW9 at UniProt or InterPro

Protein Sequence (489 amino acids)

>Pf1N1B4_5116 Sucrose alpha-glucosidase (EC 3.2.1.48) (Pseudomonas fluorescens FW300-N1B4)
MSAALEIAQHALSDGLSRVTDDYRPGYHLAPPAGWMNDPNGVVYFRGEYHVFYQHHPFDA
KWGPMYWGHAKSADLVHWQHLPIALAPGDDFDRDGCFSGSAVVCGDTLALIYTGHTWLGE
VGDERYIRQVQCLATSTDGIQFVKHGAVIDTAPQDTIMHFRDPKVWQEDDGWYLIAGARL
GDVPLLPLYRSTDLHSWEFLDYVSSGSEGDGYMWECPDLFRLNGRDVLLYSPQGMQPEGY
ERLNKYQTGYRVGRLDSQWHFTGGPFIELDNGHDFYAAQTLLAADGRRLVWAWLDMWESP
MPSQAHHWCGMLGVPRELELHADRLCVYPARELTALRMAPLPGVSWWEGSGSRWVPEVNG
DMLEIHVHLDLRGCTSGHLGIALRCSDDGHEETLLYYDASLQRLVLDRSRAGAQVTGQRS
VSIDPTQERLELRVFLDRSSIEVFDENGRFSLSSRLYPRPDSLGVKLFANGSGGRVSIPK
AWPLASGWL