Protein Info for Pf1N1B4_5113 in Pseudomonas fluorescens FW300-N1B4

Updated annotation (from data): sucrose ABC transporter, permease component 1
Rationale: Specific phenotype on sucrose
Original annotation: Maltose/maltodextrin ABC transporter, permease protein MalF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 75 to 96 (22 residues), see Phobius details amino acids 108 to 128 (21 residues), see Phobius details amino acids 134 to 153 (20 residues), see Phobius details amino acids 161 to 184 (24 residues), see Phobius details amino acids 204 to 226 (23 residues), see Phobius details amino acids 233 to 252 (20 residues), see Phobius details amino acids 264 to 286 (23 residues), see Phobius details PF00528: BPD_transp_1" amino acids 86 to 289 (204 residues), 78.4 bits, see alignment E=2.9e-26

Best Hits

Swiss-Prot: 36% identical to Y4OQ_SINFN: Probable ABC transporter permease protein y4oQ (NGR_a02190) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K02025, multiple sugar transport system permease protein (inferred from 92% identity to psp:PSPPH_5189)

Predicted SEED Role

"Maltose/maltodextrin ABC transporter, permease protein MalF" in subsystem Maltose and Maltodextrin Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A166QFV1 at UniProt or InterPro

Protein Sequence (295 amino acids)

>Pf1N1B4_5113 sucrose ABC transporter, permease component 1 (Pseudomonas fluorescens FW300-N1B4)
VRAAWLFLTPMLLCLALVAAWPLLRTFWFSLTDANLADTGGGTFIGFGNYLFHNGSSWSG
ILVDPQWWNAVRNTLYFTVVSVGLEVVLGLLVALLLNIKFTGRALVRALILIPWAIPTIV
SAKIWSWMLNDQFGIINHLMLSLGLIDAPLAWTADADLSMWAVIIVDVWKTVPFVTLLML
AALQMLPSDCYEAARVDGIHPLKVFWRVTLPLLMPALLVAAIFRILDSLRVFDVIYVLTS
NSSSTMSMSVYARQHLVEFQDVGYGSAASTLLFLVVAVIALLYLYLGRRQLEVRS